Natural history of experimental woodchuck hepatitis virus infection: molecular virologic features of the pancreas, kidney, ovary, and testis.
Natural history of experimental woodchuck hepatitis virus infection: molecular virologic features of the pancreas, kidney, ovary, and testis.
复制标题
实验性土拨鼠肝炎病毒感染的自然史：胰腺、肾脏、卵巢和睾丸的分子病毒学特征。
DOI：
10.1128/jvi.64.9.4499-4506.1990
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发表时间：
1990
影响因子：
5.4
通讯作者：
Gerin,JL
中科院分区：
文献类型：
--
作者：
Korba,BE;Brown,TL;Wells,FV;Baldwin,B;Cote,PJ;Steinberg,H;Tennant,BC;Gerin,JL
土拨鼠肝炎病毒（WHV）感染的动态模式监测胰腺，肾脏，卵巢和睾丸。在65周的时间内，从病毒感染的急性前期开始，持续到血清学恢复期或慢性感染和随后的肝细胞癌（B. E. Korba， P. J. Cote， F. V. Wells， B. Baldwin， H. Popper， R. H. Purcell， B. C. Tennant， J. L. Gerin， J. Virol. 63:1360-1370, 1989）。另外一组长期（2至3年）慢性WHV携带者的组织也接受了同样的WHV疫苗感染。在WHV感染的急性期，在所有四种组织中都发现了病毒DNA复制中间体。然而，仅在一小部分慢性感染动物的肾脏中观察到WHV DNA复制中间体。急性期感染后，WHV DNA仅存在于慢性感染土拨鼠的胰腺、肾脏和卵巢中。在这些组织中观察到与WHV复制状态相关的不同WHV基因组形式的逐步进化。这四种组织的组织学评估显示，只有极小的局部病变，与WHV活性状态无关。本研究的观察结果进一步扩展了WHV的组织亲和性。
The kinetic patterns of woodchuck hepatitis virus (WHV) infection were monitored in the pancreas, kidneys, ovaries, and testes. Groups of woodchucks experimentally infected with a standardized inoculum of WHV were sacrificed at different times over a 65-week period beginning in the preacute phase of viral infection and continuing to the period of serologic recovery or the establishment of chronic infections and subsequent hepatocellular carcinoma (B. E. Korba, P. J. Cote, F. V. Wells, B. Baldwin, H. Popper, R. H. Purcell, B. C. Tennant, and J. L. Gerin, J. Virol. 63:1360-1370, 1989). Tissues from an additional group of long-term (2 to 3 years) chronic WHV carriers which had been infected with the same WHV inocula were also examined. Viral DNA replication intermediates were found in all four tissues during the acute phase of WHV infection. However, WHV DNA replication intermediates were observed only in the kidneys of a small proportion of the chronically infected animals. Following the acute phase of infection, WHV DNA was present only in the pancreas, kidneys, and ovaries of the chronically infected woodchucks. A progressive evolution of different WHV genomic forms related to the replicative state of WHV was observed in these tissues. Histologic evaluation of these four tissues revealed only minimal, localized lesions which were not correlated with the state of WHV activity. The observations compiled in this study further extend the tissue tropism of WHV.