Lipase production and Penicillium simplicissimum morphology in solid-state and submerged fermentations
Lipase production and Penicillium simplicissimum morphology in solid-state and submerged fermentations
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DOI：
10.1002/biot.200800298
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发表时间：
2009-10-01
影响因子：
4.7
通讯作者：
Guimaraes Freire, Denise Maria
中科院分区：
文献类型：
--
作者：
Estrada Gutarra, Melissa Limoeiro;de Godoy, Mateus Gomes;Guimaraes Freire, Denise Maria
比较研究了简青霉固态发酵和深层发酵的形态和脂肪酶产量。SSF进行巴巴苏饼作为培养基和SmF的半合成培养基和悬浮巴巴苏饼颗粒的基础上的培养基。在SSF中，生物量、比活和分生孢子产量的产物产量分别高3.3倍、1.3倍和2倍。在SmF中，真菌生长的类型根据培养基而不同。使用半合成培养基，真菌形成密集交织的菌丝体，而不产生分生孢子，而使用基于巴巴苏的培养基，真菌形成游离菌丝体并粘附于谷粒的表面，产生分生孢子。结果表明，巴巴苏蛋糕诱导产孢的SmF。在SSF中，真菌不仅生长在颗粒表面，产生大量的分生孢子，而且还能有效地定殖和穿透巴巴苏颗粒。在SSF中的高分生孢子产量和脂肪酶生产率可能与营养物的低可用性或与这种类型的发酵相关的其他刺激有关。因此，使用非补充的、低成本的农业工业残余物作为培养基，热稳定的P.simplicissimum脂肪酶的高产量证明了SSF用于生产工业酶的生物技术潜力。
A comparative study of Penicillium simplicissimum morphology and lipase production was performed using solid-state (SSF) and submerged (SmF) fermentation. SSF was carried out on babassu cake as culture medium and SmF on a semi-synthetic medium and a medium based on suspended babassu cake grains. Yield of product on biomass, specific activity and conidia production were 3.3-, 1.3- and 2-fold higher in SSF. In SmF, the type of fungus growth differed according to the medium. Using the semi-synthetic medium, the fungus formed densely interwoven mycelial masses without conidia production, whereas using the babassu-based medium the fungus formed free mycelia and adhered to the surfaces of the grains, producing coniclia. The results show that babassu cake induces conidiation in SmF. In SSF, the fungus not only grew on the surface of the grains, producing coniclia abundantly, but also effectively colonized and penetrated the babassu particles. The high coniclia production and lipase productivity in SSF may be related to the low availability of nutrients or to other stimuli associated with this type of fermentation. Thus, the high production of the thermostable P. simplicissimum lipase, using a non-supplemented, low-cost agro-industrial residue as the culture medium, demonstrates the biotechnological potential of SSF for the production of industrial enzymes.