Effect of Resveratrol on Modulation of Endothelial Cells and Macrophages for Rapid Vascular Regeneration from Electrospun Poly(epsilon-caprolactone) Scaffolds
Effect of Resveratrol on Modulation of Endothelial Cells and Macrophages for Rapid Vascular Regeneration from Electrospun Poly(epsilon-caprolactone) Scaffolds
复制标题
白藜芦醇对电纺聚（ε-己内酯）支架内皮细胞和巨噬细胞快速血管再生的调节作用。
DOI：
10.1021/acsami.6b16573
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发表时间：
2017
影响因子：
9.5
通讯作者：
Kong Deling
中科院分区：
文献类型：
--
作者：
Wang Zhihong;Wu Yifan;Wang Jianing;Zhang Chuangnian;Yan Hongyu;Zhu Meifeng;Wang Kai;Li Chen;Xu Qingbo;Kong Deling
快速内皮化是决定小直径血管移植物作为动脉替代物治疗心血管疾病成功的关键因素。为了促进血管再生，我们开发了一种载有白藜芦醇的血管支架，白藜芦醇是一种从植物中提取的天然化合物，在心血管保护方面表现出多方面的作用。采用白藜芦醇在聚己内酯（PCL）溶液中静电纺丝制备管状聚己内酯（PCL）支架。体外释放实验表明，白藜芦醇可以从支架中持续、可控地释放。细胞培养结果表明，与PCL对照组相比，含白藜芦醇的PCL支架组的内皮细胞（EC）的迁移，一氧化氮的产生和管形成的能力增加。同时，含白藜芦醇组巨噬细胞分泌的主要促炎因子肿瘤坏死因子（TNF）-α水平降低，M2巨噬细胞相关基因mRNA表达增加。此外，通过置换大鼠腹主动脉进行体内植入。我们观察到快速内皮化和增强血管再生大鼠含白藜芦醇的支架。白藜芦醇的存在也诱导了大量的M2巨噬细胞浸润到移植物壁中。总之，白藜芦醇掺入PCL移植物通过调节EC和巨噬细胞来增强血管再生。
Rapid endothelialization is a key factor that determines the success of small-diameter vascular grafts as an artery substitute in the treatment of cardiovascular diseases. Aimed to facilitate vascular regeneration, we developed a vascular scaffold loaded with resveratrol, which is a natural compound extracted from plants and showed multifaceted effects in cardiovascular protection. The tubular poly(ε-caprolactone) (PCL) scaffold was prepared by electrospinning with resveratrol in the PCL solution. In vitro assay demonstrated that resveratrol could be released from the scaffolds in a sustained and controlled manner. Cell culture results indicated that the migration of endothelial cells (ECs), nitric oxide production, and the ability of tube formation increased in the resveratrol-containing PCL scaffold groups compared with the PCL control. Meanwhile, the level of tumor necrosis factor (TNF)-α, the main proinflammatory factor secreted from macrophages, was reduced, and the messenger RNA expressions of the M2 macrophage-related genes were increased in the resveratrol-containing group. Further, in vivo implantation was performed by replacing rat abdominal aorta. We observed fast endothelialization and enhanced vascular regeneration in rats with resveratrol-containing scaffolds. The presence of resveratrol also induced a large number of M2 macrophages to infiltrate into the graft wall. Taken together, the incorporation of resveratrol into the PCL grafts enhanced the vascular regeneration by modulation of ECs and macrophages.