gpUL73 (gN) genomic variants of human cytomegalovirus isolates are clustered into four distinct genotypes
gpUL73 (gN) genomic variants of human cytomegalovirus isolates are clustered into four distinct genotypes
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DOI：
10.1099/0022-1317-82-11-2777
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发表时间：
2001-11-01
影响因子：
3.8
通讯作者：
Landini, MP
中科院分区：
文献类型：
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作者：
Pignatelli, S;Dal Monte, P;Landini, MP
人类巨细胞病毒 (HCMV) 的临床分离株在组织趋向性、临床表现的严重程度以及建立持续或潜伏感染的能力方面表现出差异，这些特征被认为与毒株之间的基因组变异有关。这项工作分析了新的 HCMV 多态性基因座、开放阅读框 (ORF) UL73 的基因组变异。该ORF编码包膜糖蛋白gpUL73 (gN)，其在高分子质量复合物中与其对应物gM结合，并在宿主中诱导中和抗体反应。对临床分离株和实验室适应株的 ORF UL73 及其基因产物的详细序列分析表明，这种糖蛋白具有高度多态性，特别是在 N 末端区域。 gpUL73 高变异性不是随机分布的，但已识别的基因组变异明显聚集为四种不同的基因型（gN-1、gN-2、gN-3 和 gN-4），这些基因型与 gB 亚型无关。
Clinical isolates of human cytomegalovirus (HCMV) show differences in tissue tropism, severity of clinical manifestations and ability to establish persistent or latent infections, characteristics that are thought to be related to genomic variation among strains. This work analysed the genomic variants of a new HCMV polymorphic locus, open reading frame (ORF) UL73. This ORF encodes the envelope glycoprotein gpUL73 (gN), which associates in a high molecular mass complex with its counterpart, gM, and induces a neutralizing antibody response in the host. Detailed sequence analysis of ORF UL73 and its gene product from clinical isolates and laboratory-adapted strains shows that this glycoprotein is highly polymorphic, in the N-terminal region in particular. gpUL73 hypervariability is not randomly distributed, but the identified genomic variants are clearly clustered into four distinct genotypes (gN-1, gN-2, gN-3 and gN-4), which are not associated with the gB subtype.