TLR9 stimulation drives naive B cells to proliferate and to attain enhanced antigen presenting function
TLR9 stimulation drives naive B cells to proliferate and to attain enhanced antigen presenting function
复制标题
DOI：
10.1002/eji.200636984
复制
发表时间：
2007-08-01
影响因子：
5.4
通讯作者：
Sieg, Scott F.
中科院分区：
文献类型：
--
作者：
Jiang, Wei;Lederman, Michael M.;Sieg, Scott F.
调节幼稚B细胞增殖和功能的机制尚未完全确定。在这项研究中，我们测试的假设，幼稚B细胞的扩增，生存和抗原呈递给T淋巴细胞的能力可以直接调节Toll样受体（TLR）激动剂。在没有B细胞受体刺激的情况下，CpG寡核苷酸（一种TLR 9激动剂）在诱导幼稚B细胞增殖和存活方面特别有效。尽管扩增的初始B细胞并未成熟为CD 27（+）或IgG（+）记忆B细胞，但这些细胞确实分化为HLA-DR、CD 40和CD 80表面表达增加的IgM分泌细胞。这与这些B细胞激活同种异体T细胞的潜力增加有关。我们提出，由TLR 9激动剂诱导的幼稚B细胞的活化和扩增可以增强这些细胞与同源抗原相互作用的潜力，并促进细胞介导的免疫应答。
Mechanisms that regulate naive B cell proliferation and function are incompletely defined. In this study, we test the hypothesis that naive B cell expansion, survival and ability to present antigen to T lymphocytes can be directly modulated by Toll-like receptor (TLR) agonists. In the absence of B cell receptor stimulation, CpG oligonucleotide, a TLR9 agonist, was particularly efficient in inducing naive B cell proliferation and survival. Although the expanded naive B cells did not mature into CD27(+) or IgG(+) memory B cells, these cells did differentiate into IgM-secreting cells with increased surface expression of HLA-DR, CD40 and CD80. This was associated with an increased potential for these B cells to activate allogeneic T cells. We propose that the activation and expansion of naive B cells induced by TLR9 agonists could enhance the potential of these cells to interact with cognate antigens and facilitate cell-mediated immune responses.