Measuring interstitial fluid pressure with fiberoptic pressure transducers
Measuring interstitial fluid pressure with fiberoptic pressure transducers
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DOI：
10.1016/j.mvr.2008.08.002
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发表时间：
2009-03-01
影响因子：
3.1
通讯作者：
Ozerdem, Ugur
中科院分区：
文献类型：
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作者：
Ozerdem, Ugur
在这份报告中，我们描述了一种使用基于光学干涉测量的光纤压力传感器测量间质流体压力(IFP)的实用方法。使用FOBPS-18光纤压力传感器，8只小鼠用于皮下IFP测量，4只小鼠用于肌肉内IFP测量。我们用Samba-420磁共振光纤压力传感器测量了四只小鼠的皮下IFP。用光纤系统和建立的方法同时对每只小鼠进行一次测量。在皮下组织中，FOBPS光纤换能器、Samba光纤换能器的平均IFP值分别为-3.00 mm Hg(Sm-/+0.462，n=8)、-3.25 mm Hg(Sm-/+0.478，n=4)、-3.34 mm Hg(Sm-/+0.312，n=6)和-2.85(Sm-/+0.57，n=6)。分别采用换能器尖端导管和针芯穿刺法。测量IFP的这些技术之间没有差异(Friedman检验，p=0.7997)。皮下IFP测量结果显示，光纤传感器与传感器尖端导管(R(2)=0.9950)和光纤传感器与针芯技术(R(2)=0.9966)之间具有很强的线性相关性。光纤压力传感器准确地测量间质液体压力，与传统技术相当。本报告中描述的简化的IFP测量程序将使研究人员能够轻松地测量IFP，并阐明在MRI中遇到强电磁场时正常组织或肿瘤组织中单位体积变化的单位压力变化(dp/dv)。(C)2008 Elsevier Inc.保留所有权利。
In this report we describe a practical procedure for measuring interstitial fluid pressure (IFP) using fiberoptic pressure transducers based on optical interferometry. Eight mice were used for subcutaneous IFP measurements and four mice for intramuscular IFP measurements with a FOBPS-18 fiberoptic pressure transducer. We used four mice for subcutaneous IFP measurements with a SAMBA-420 MR fiberoptic pressure transducer. One measurement was made for each mouse simultaneously by using a fiberoptic system and an established approach, either transducer-tipped catheter or wick-in-needle technique.The mean IFP values obtained in subcutaneous tissues were -3.00 mm Hg (SEM -/+ 0.462, n = 8), -3.25 mm Hg (SEM -/+ 0.478, n = 4), - 3.34 mm Hg (SEM -/+ 0.312, n = 6), and -2.85 (SEM -/+ 0.57, n = 6) for the FOBPS fiberoptic transducer, the SAMBA fiberoptic transducer, the transducer-tipped catheter, and the wick-in-needle technique, respectively. There was no difference between these techniques to measure IFP (Friedman test, p=0.7997). The subcutaneous IFP measurements showed strong linear correlation between fiberoptic transducer and transducer-tipped catheter (R(2)=0.9950) and fiberoptic transducer and wick-in-needle technique (R(2)=0.9966).Fiberoptic pressure transducers measure the interstitial fluid pressure accurately, comparable to conventional techniques. The simplified IFP measurement procedures described in this report will allow investigators to easily measure IFP, and elucidate the unit pressure change per unit volume change (dP/dV) in normal or cancer tissues in the presence of strong electromagnetic fields encountered in MRI. (c) 2008 Elsevier Inc. All rights reserved.