Connexin36 mediates gap junctional coupling of alpha‐ganglion cells in mouse retina
Connexin36 mediates gap junctional coupling of alpha‐ganglion cells in mouse retina
复制标题
Connexin36介导小鼠视网膜α神经节细胞的间隙连接偶联
DOI：
10.1002/cne.20510
复制
发表时间：
2005
影响因子：
2.5
通讯作者：
R. Weiler
中科院分区：
文献类型：
--
作者：
T. Schubert;Joachim Degen;K. Willecke;S. Hormuzdi;H. Monyer;R. Weiler
α神经节细胞存在于所有脊椎动物的视网膜中，并根据其内丛状层内树突分枝的水平细分为ON和OFF类型。它们具有较大的树突域，通常对移动刺激具有良好的响应能力。它们是第一个观察到示踪剂耦合的神经节细胞，表明存在由未知连接蛋白组成的间隙连接。在这里，我们发现小鼠视网膜中的ON-α-神经节细胞与无长突细胞偶联，而OFF-α-神经节细胞与其他OFF-α-神经节细胞和无长突细胞偶联。这些示踪剂耦合模式在连接蛋白 36 (Cx36) 缺陷的小鼠中完全不存在。在 Cx36 编码 DNA 被 lacZ 报告基因取代的小鼠中，Cx36 蛋白在 α 神经节细胞中表达，但在偶联无长突细胞中不表达。在将增强型绿色荧光蛋白（EGFP）连接到 Cx36 蛋白 C 末端区域的小鼠中，对 Cx36 斑块的树突定位和分布模式进行了分析，结果显示荧光斑块数量相当少，并且 ON 和 OFF α 神经节细胞的模式不同。此外，OFF-α-神经节细胞之间的示踪剂耦合可以被奎宁抑制，奎宁是一种间隙连接阻断剂，对 Cx36 形成的间隙连接略有偏好。这些数据强烈表明，Cx36 间隙连接通道不仅在中间神经元中起作用，而且在视网膜的输出神经元中也起作用，并且负责神经节细胞的不同耦合模式。 J.Comp。内罗尔。 485:191–201, 2005。© 2005 Wiley-Liss, Inc.
Alpha‐ganglion cells are present in all vertebrate retinae and are subdivided into ON and OFF types according to their level of dendritic ramification within the inner plexiform layer. They have large dendritic fields and usually a good responsiveness to moving stimuli. They were the first ganglion cells in which tracer coupling was observed, suggesting the presence of gap junctions composed of unknown connexins. Here we show that ON‐alpha‐ganglion cells in the mouse retina are coupled to amacrine cells, whereas OFF‐alpha‐ganglion cells are coupled to other OFF‐alpha‐ganglion cells and to amacrine cells. These tracer coupling patterns were completely absent in mice deficient in connexin36 (Cx36). The expression of Cx36 protein in alpha‐ganglion cells but not in coupled amacrine cells was confirmed in mice in which the Cx36 coding DNA was replaced by the lacZ reporter gene. The dendritic localization and the distribution pattern of Cx36 patches, analyzed in mice in which the enhanced green fluorescent protein (EGFP) was linked to the C‐terminal region of the Cx36 protein, revealed a rather small number of fluorescent plaques and different patterns for ON‐ and OFF‐alpha‐ganglion cells. Furthermore, tracer coupling between OFF‐alpha‐ganglion cells could be inhibited by quinine, a gap junctional blocker with a slight preference for gap junctions formed by Cx36. These data strongly suggest that Cx36 gap junction channels are functional not only in interneurons but also in output neurons of the retina and are responsible for distinct coupling patterns of ganglion cells. J. Comp. Neurol. 485:191–201, 2005. © 2005 Wiley‐Liss, Inc.
DOI：
10.1073/pnas.97.13.7573
发表时间：
2000-06-20
影响因子：
11.1
作者：
Rash, JE;Staines, WA;Nagy, JI
通讯作者：
Nagy, JI
影响因子：
2.5
作者：
DeVries, SH
通讯作者：
DeVries, SH