Initial state of the Drosophila eye before dorsoventral specification is equivalent to ventral
Initial state of the Drosophila eye before dorsoventral specification is equivalent to ventral
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DOI：
10.1242/dev.00864
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Choi, KW
中科院分区：
文献类型：
--
作者：
Singh, A;Choi, KW
背腹(DV)模式对无脊椎动物和高等动物的眼睛发育至关重要。DV谱系限制是果蝇未分化早期眼原基的主要事件。在果蝇眼盘里。背部特异的GATA家族转录因子pannier(PnR)控制易洛魁复合体(IRO-C)基因决定眼睛背侧的命运，而叶(L)参与控制Notch配体Serrate(Serrate)，是腹面生长所特需的。然而，眼盘细胞在PNR和IRO-C开始背部表达之前的命运尚不清楚。我们发现，L/丝氨酸在整个早期眼盘中都有表达，而在一龄后期的背侧眼缘细胞中，PNR和IRO-C才开始表达。我们的证据表明，在胚胎发育过程中，PNR活性对眼睛发育并不是必需的。我们提供的证据表明，在PNR表达开始之前，L或丝氨酸功能的丧失会导致整个眼睛的消失，而在PNR表达开始之后，它只会导致优先失去半只眼的腹侧。我们证明，当去除PnR或iro-C基因功能后，背侧眼盘细胞也变得对L或丝氨酸依赖。因此，我们认为DV谱系限制之前眼睛的早期状态相当于腹侧，需要L和丝氨酸基因的功能。
Dorsoventral (DV) patterning is crucial for eye development in invertebrates and higher animals. DV lineage restriction is the primary event in undifferentiated early eye primordia of Drosophila. In Drosophila eye disc. a dorsal-specific GATA family transcription factor pannier (pnr) controls Iroquois-Complex (Iro-C) genes to establish the dorsal eye fate whereas Lobe (L), which is involved in controlling a Notch ligand Serrate (Ser), is specifically required for ventral growth. However, fate of eye disc cells before the onset of dorsal expression of pnr and Iro-C is not known. We show that L/Ser are expressed in entire early eye disc before the expression of pnr and Iro-C is initiated in late first instar dorsal eye margin cells. Our evidence suggests that during embryogenesis pnr activity is not essential for eye development. We present evidence that loss of L or Ser function prior to initiation of pnr expression results in elimination of the entire eye, whereas after the onset of pnr expression it results only in preferential loss of ventral half of eye. We demonstrate that dorsal eye disc cells also become L or Ser dependent when they are ventralized by removal of pnr or Iro-C gene function. Therefore, we propose that early state of the eye prior to DV lineage restriction is equivalent to ventral and requires L and Ser gene function.