Experimental spinal fusion with use of recombinant human bone morphogenetic protein 2
Experimental spinal fusion with use of recombinant human bone morphogenetic protein 2
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DOI：
10.1097/00007632-199907150-00003
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发表时间：
1999-07-15
期刊：
影响因子：
3
通讯作者：
Takaoka, K
中科院分区：
文献类型：
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作者：
Itoh, H;Ebara, S;Takaoka, K
研究设计.通过植入重组人骨形态发生蛋白-2（rhBMP-2）与胶原载体复合物，观察rhBMP-2在兔腰椎后外侧融合中的作用。检测rhBMP-2的骨形成诱导活性，并找到rhBMP加入胶原载体中的最佳量，以构成骨形成诱导植入物，用于在兔后外侧脊柱融合术中替代骨移植物。在动物模型中，rhBMP-2浸渍的胶原蛋白已成功用于后外侧脊柱融合，表明其是目前最常规用于后外侧腰椎融合的自体皮质松质骨移植物的潜在替代品。将9只家兔分为3组。暴露双侧L4-L5横突，将浸渍有rhBMP-2（10、50或200 μ g）的胶原条置于左侧横突上，并将单独的胶原条插入右侧。术后24周处死动物。通过X线片和组织学检查评估植入部位的新骨形成和骨融合情况。除1只（10 μ g rhBMP-2）外，所有家兔左侧横突间均观察到新骨形成。植入12周后，所有动物右侧未见新骨形成。50 μ g和200 μ g rhBMP-2组的新骨形成量显著大于10 μ g rhBMP-2组（P < 0.01），但50 μ g和200 μ g rhBMP-2组的骨形成量无显著差异（P = 0.647）。rhBMP-2/胶原复合植入物是实现脊柱后外侧融合的有效骨移植替代物。当与胶原蛋白载体结合时，在兔中实现后外侧脊柱融合的最佳rhBMP-2剂量似乎为每节段约50 μ g。
Study Design. Posterolateral lumbar spinal fusion with use of recombinant human bone morphogenetic protein 2 (rhBMP-P) was tested in rabbits by implanting composites of rhBMP-2 and collagen carrier.Objectives. To examine the bone-formation-inducing activity of rhBMP-2 and find the optimal amount of rhBMP to add to a collagen carrier to constitute bone-formation-inducing implants to be substituted for bone graft in posterolateral spinal fusion in rabbits.Summary of Background Data. In animal models, rhBMP-2-impregnated collagen has been successfully used for posterolateral spinal fusion, indicating that it is a potential substitute for the autogenous corticocancellous bone graft currently used most routinely in posterolateral lumbar spinal fusion.Methods. Nine rabbits were divided into three equal groups. The bilateral L4-L5 transverse processes were exposed, and collagen strips impregnated with rhBMP-2 (10, 50, or 200 mu g) were placed on the left transverse processes, and collagen strips alone were inserted on the right. All rabbits were killed 24 weeks after surgery. The implanted sites were assessed for new bone formation and bony fusion by radiography and histologic examination.Results. New bone formation was noted in intertransverse spaces on the left side of all rabbits except one (10 mu g rhBMP-2). Twelve weeks after implantation, no new bone formation was seen on the right side of all animals. The newly formed bone masses were significantly larger in the 50-mu g and 200-mu g rhBMP-2 groups than in the 10-mu g rhBMP-2 group (P < 0.01), but there was no significant difference between bone formation in the 50-mu g and 200-mu g groups (P = 0.647).Conclusions. The rhBMP-2/collagen composite implant was an effective bone graft substitute for achieving posterolateral spinal fusion. When combined with a collagen carrier, the optimal rhBMP-2 dose for achieving posterolateral spinal fusion seemed to be approximately 50 mu g per segment in rabbits.