Involvement of fibroblast growth factor (FGF)18-FGF8 signaling in specification of left-right asymmetry and brain and limb development of the chick embryo
Involvement of fibroblast growth factor (FGF)18-FGF8 signaling in specification of left-right asymmetry and brain and limb development of the chick embryo
复制标题
DOI：
10.1016/s0925-4773(00)00331-2
复制
发表时间：
2000-07-01
影响因子：
2.6
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Ohuchi, H;Kimura, S;Itoh, N
为了阐明成纤维细胞生长因子 (FGF)18 在脊椎动物发育过程中的作用，我们检查了鸡胚中 Fgf18 的表达模式，并观察了 FGF18 蛋白对 Hensen 结、峡部和肢芽的影响。 Fgf18在Fgf8表达开始之前的节点右侧表达。 FGF18 蛋白可以诱导 Fgf8 在节点左侧表达，表明两种 FGF 都参与了左右不对称的规范。在发育中的大脑中，继 Fgf8 表达之后，Fgf18 在峡部表达。由于 Fgf18 在第二中脑形成过程中由 FGF8 蛋白异位诱导，因此两种 FGF 也能促进中脑发育。在肢芽中，FGF18 在间充质中表达，FGF18 蛋白的异位应用抑制了肢体中的骨生长。因此，FGF18 可能是 FGF 受体 3 的内源性配体，其突变会导致人类骨发育不良。这些结果表明 FGF18-FGF8 信号传导参与各种组织活动，并且 FGF18 和 FGF8 之间的信号传导层次似乎在不同结构的模式化过程中发生变化。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
To elucidate roles of fibroblast growth factors (FGF)18 during vertebrate development, we examined expression patterns of Fgf18 in chick embryos and observed effects of FGF18 protein on the Hensen's node, isthmus, and limb buds. Fgf18 is expressed on the right side of the node before the expression of Fgf8 starts. FGF18 protein can induce expression of Fgf8 on the left side of the node, indicating involvement of both FGFs in specification of left-right asymmetry. In the developing brain, Fgf18 is expressed in the isthmus, following the Fgf8 expression. Since Fgf18 is induced ectopically during formation of the second midbrain by FGF8 protein, both FGFs also elaborate midbrain development. In the limb bud, Fgf18 is expressed in the mesenchyme and ectopic application of FGF18 protein inhibits bone growth in the limb. FGF18 is thus likely an endogenous ligand of FGF receptor 3, whose mutation causes bone dysplasia in humans. These results demonstrate that the FGF18-FGF8 signaling is involved in various organizing activities and the signaling hierarchies between FGF18 and FGF8 seem to change during patterning of different structures. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.