Bioactive components, antioxidant and antimicrobial activities of Paeonia rockii fruit during development
Bioactive components, antioxidant and antimicrobial activities of Paeonia rockii fruit during development
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DOI：
10.1016/j.foodchem.2020.128444
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发表时间：
2021-05-01
期刊：
影响因子：
8.8
通讯作者：
Zhang, Yan-Long
中科院分区：
文献类型：
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作者：
Bai, Zhang-Zhen;Ni, Jing;Zhang, Yan-Long
近十年来，尽管牡丹果实具有其他潜在的利用潜力，但其用于食用油的研究却备受关注。本研究采用液相色谱-电喷雾电离-三重四极杆质谱（LC-ESI-QqQ-MS）靶向方法对TPF开发过程中的29种生物活性成分进行了鉴定和定量。通过主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA），筛选出反式白藜芦醇、苯甲酸、木犀草素和没食子酸甲酯作为种子和荚果间的优势化学标记。在第2期（S2）和第6期种子中，芍药苷（1893 mg/100 g）和反式白藜芦醇（1793 mg/100 g）的含量分别极高。ABTS（+中心点）、DPPH中心点和FRAP测定的抗氧化活性与总酚含量（TPC）和总黄酮含量（TFC）呈显著相关。豆荚和种子对金黄色葡萄球菌和寻常变形杆菌的抑菌作用在萌芽期和成熟期最强。TPF可能是具有功能特性的生物活性化合物的潜在来源。
In last ten years, much attention focused on tree peony fruit (TPF) for edible oil production despite other potential utilization. The present study identified and quantified 29 bioactive components by liquid chromatography-electrospray ionization-triple quadrupole-mass spectrometry (LC-ESI-QqQ-MS) targeted approach during the development of TPF. Trans-resveratrol, benzoic acid, luteolin, and methyl gallate were selected as predominant chemical markers between seeds and pods through principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA). Extremely high levels of paeoniflorin (1893 mg/100 g) and trans-resveratrol (1793 mg/100 g) were observed at stage 2 (S2) and S6 in seeds, respectively. Antioxidant activities determined by ABTS(+center dot), DPPH center dot, and FRAP assays showed significant correlations with total phenolic content (TPC) and total flavonoid content (TFC). The strongest antibacterial effects of pod and seed against Staphylococcus aureus and Proteus vulgaris occurred at initial stages and maturation stages. TPF could be a potential source of bioactive compounds with functional properties.