Secondary structure, backbone dynamics, and structural topology of phospholamban and its phosphorylated and Arg9Cys-mutated forms in phospholipid bilayers utilizing 13C and 15N solid-state NMR spectroscopy.
Secondary structure, backbone dynamics, and structural topology of phospholamban and its phosphorylated and Arg9Cys-mutated forms in phospholipid bilayers utilizing 13C and 15N solid-state NMR spectroscopy.
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DOI：
10.1021/jp500316s
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发表时间：
2014-02-27
期刊：
影响因子：
--
通讯作者：
Lorigan GA
中科院分区：
文献类型：
--
作者：
Yu X;Lorigan GA
受磷蛋白（PLB）是一种膜蛋白，通过与肌浆网Ca 2 + ATP酶（SERCA）相互作用调节心肌舒张速率。当PLB被磷酸化或Arg 9 Cys（R9 C）突变时，SERCA的抑制被解除。利用13 C和15 N固态NMR光谱研究PLB在磷酸化和R9 C突变后的构象变化。胞质结构域的13 C =O NMR光谱揭示了两种α-螺旋结构组分，其在磷酸化和R9 C突变后具有群体变化。在结构域Ib上观察到非结构化成分的出现。15 N NMR光谱表明细胞质结构域的骨架动力学增加。野生型PLB（WT-PLB）、Ser 16-磷酸化PLB（P-PLB）和R9 C-突变的PLB（R9 C-PLB）都具有非常动态的结构域Ib，并且跨膜结构域具有固定的组分。15 N NMR谱表明，R9 C-PLB的胞质结构域采用类似于P-PLB的取向，并远离膜表面移动。P-PLB和R9 C-PLB的结构域Ib（Leu 28）失去比对。R9 C-PLB采用类似于P-PLB的构象，具有向更扩展和无序状态的群体转变。NMR数据表明PLB的更广泛和无序形式可能与抑制缓解有关。
Phospholamban (PLB) is a membrane protein that regulates heart muscle relaxation rates via interactions with the sarcoplasmic reticulum Ca2+ ATPase (SERCA). When PLB is phosphorylated or Arg9Cys (R9C) is mutated, inhibition of SERCA is relieved. 13C and 15N solid-state NMR spectroscopy is utilized to investigate conformational changes of PLB upon phosphorylation and R9C mutation. 13C=O NMR spectra of the cytoplasmic domain reveal two α-helical structural components with population changes upon phosphorylation and R9C mutation. The appearance of an unstructured component is observed on domain Ib. 15N NMR spectra indicate an increase in backbone dynamics of the cytoplasmic domain. Wild-type PLB (WT-PLB), Ser16-phosphorylated PLB (P-PLB), and R9C-mutated PLB (R9C-PLB) all have a very dynamic domain Ib, and the transmembrane domain has an immobile component. 15N NMR spectra indicate that the cytoplasmic domain of R9C-PLB adopts an orientation similar to P-PLB and shifts away from the membrane surface. Domain Ib (Leu28) of P-PLB and R9C-PLB loses the alignment. The R9C-PLB adopts a conformation similar to P-PLB with a population shift to a more extended and disordered state. The NMR data suggest the more extended and disordered forms of PLB may relate to inhibition relief.
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影响因子：
3.4
作者：
Chu, Shidong;Coey, Aaron T.;Lorigan, Gary A.
通讯作者：
Lorigan, Gary A.
影响因子：
3.3
作者：
Hong, Mei
通讯作者：
Hong, Mei
影响因子：
5.6
作者：
Gustavsson, Martin;Traaseth, Nathaniel J.;Veglia, Gianluigi
通讯作者：
Veglia, Gianluigi
Phospholamban 突变体与野生型竞争活细胞中的 SERCA 结合。
DOI：
10.1016/j.bbrc.2012.02.125
发表时间：
2012-04-06
影响因子：
3.1
作者：
Gruber SJ;Haydon S;Thomas DD
通讯作者：
Thomas DD
影响因子：
4.8
作者：
Chen, Zhenhui;Akin, Brandy L.;Jones, Larry R.
通讯作者：
Jones, Larry R.