Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels
Effects of Overproduction of Rubisco Activase on Rubisco Content in Transgenic Rice Grown at Different N Levels
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DOI：
10.3390/ijms21051626
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Makino, Amane
中科院分区：
文献类型：
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作者：
Suganami, Mao;Suzuki, Yuji;Makino, Amane
据报道，水稻 (Oryza sativa L.) 中 Rubisco 激活酶 (RCA) 的过量产生降低了 Rubisco 含量，导致光合作用下降。我们使用在 RCA 和 Rubisco 小亚基 (RBCS) 基因的不同启动子控制下过表达或抑制 RCA 的转基因水稻，研究了 RCA 水平对 Rubisco 含量的影响。所有植物均采用不同氮浓度（0.5、2.0 和 8.0 mM-N）进行水培。在RCA含量> 2倍的RCA过量生产的植物（RCA-HI品系）中，在0.5和2.0 mM-N下观察到Rubisco含量下降10%-20%。相反，在 8.0 mM-N 时，RCA-HI 系中的 Rubisco 含量没有变化。相反，在 RCA 含量增加 50%-60% 的植物（RCA-MI 系）中，无论氮浓度如何，Rubisco 水平都保持不变。这种对 Rubisco 含量的影响与所使用的启动子无关。在 RCA 抑制<野生型 RCA 含量 10% 的植物中，Rubisco 水平在 0.5 mM-N 时增加，但在 2.0 和 8.0 mM-N 时保持不变。因此，RCA 水平变化对 Rubisco 含量的影响取决于氮的供应。此外，在不降低 Rubisco 含量的情况下，RCA 过量生产是可行的，具体取决于 RCA 生产的程度。
It has been reported that overproduction of Rubisco activase (RCA) in rice (Oryza sativa L.) decreased Rubisco content, resulting in declining photosynthesis. We examined the effects of RCA levels on Rubisco content using transgenic rice with overexpressed or suppressed RCA under the control of different promoters of the RCA and Rubisco small subunit (RBCS) genes. All plants were grown hydroponically with different N concentrations (0.5, 2.0 and 8.0 mM-N). In RCA overproduced plants with > 2-fold RCA content (RCA-HI lines), a 10%-20% decrease in Rubisco content was observed at 0.5 and 2.0 mM-N. In contrast, at 8.0 mM-N, Rubisco content did not change in RCA-HI lines. Conversely, in plants with 50%-60% increased RCA content (RCA-MI lines), Rubisco levels remained unchanged, regardless of N concentration. Such effects on Rubisco content were independent of the promoter that was used. In plants with RCA suppression to < 10% of the wild-type RCA content, Rubisco levels were increased at 0.5 mM-N, but were unchanged at 2.0 and 8.0 mM-N. Thus, the effects of the changes in RCA levels on Rubisco content depended on N supply. Moreover, RCA overproduction was feasible without a decrease in Rubisco content, depending on the degree of RCA production.