Plant Cell Growth Responds to External Forces and the Response Requires Intact Microtubules
Plant Cell Growth Responds to External Forces and the Response Requires Intact Microtubules
复制标题
植物细胞生长对外力做出反应，而这种反应需要完整的微管
作者：
C. Wymer;S. Wymer;D. Cosgrove;R. Cyr
大多数植物细胞中的微纤丝沉积受皮层微管的影响。因此，皮层微管是为细胞壁提供空间信息的模板。皮质微管如何获得它们的空间信息和定位是未知的。有迹象表明，植物细胞通过使用微管作为传感元件来响应机械应力。再生原生质体从烟草（烟草）用于确定是否可以诱导细胞扩大在一个优先的方向，以响应外部施加的单向力。此外，使用抗微管除草剂来研究微管在对这种力的响应中的作用。将原生质体包埋在琼脂糖中，以28至34 g短暂离心，并培养或立即制备用于其微管的免疫定位。许多离心原生质体内的微管被发现是平行于离心力矢量取向。大多数原生质体伸长的优先轴是定向60至90[度]所施加的力矢量。瞬时处理的原生质体与可逆的微管破坏剂amiprostin-甲基（应用之前和离心过程中）延长，但没有一个优先的生长轴。这些结果表明，短暂的生物物理力可能会影响皮层微管的排列，微管本身作为生物物理响应元素。
Microfibril deposition in most plant cells is influenced by cortical microtubules. Thus, cortical microtubules are templates that provide spatial information to the cell wall. How cortical microtubules acquire their spatial information and are positioned is unknown. There are indications that plant cells respond to mechanical stresses by using microtubules as sensing elements. Regenerating protoplasts from tobacco (Nicotiana tabacum) were used to determine whether cells can be induced to expand in a preferential direction in response to an externally applied unidirectional force. Additionally, an anti-microtubule herbicide was used to investigate the role of microtubules in the response to this force. Protoplasts were embedded in agarose, briefly centrifuged at 28 to 34g, and either cultured or immediately prepared for immunolocalization of their microtubules. The microtubules within many centrifuged protoplasts were found to be oriented parallel to the centrifugal force vector. Most protoplasts elongated with a preferential axis that was oriented 60 to 90[deg] to the applied force vector. Protoplasts treated transiently with the reversible microtubule-disrupting agent amiprophos-methyl (applied before and during centrifugation) elongated but without a preferential growth axis. These results indicate that brief biophysical forces may influence the alignment of cortical microtubules and that microtubules themselves act as biophysical responding elements.
激光显微手术对分离植物原生质体中细胞质链和细胞质流的影响。
影响因子：
6.6
作者：
Hahne,G;Hoffmann,F
通讯作者：
Hoffmann,F