Expression of TRPV1 in the C57BL/6 mice brain hippocampus and cortex during development
Expression of TRPV1 in the C57BL/6 mice brain hippocampus and cortex during development
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TRPV1在C57BL/6小鼠发育过程中大脑海马和皮质中的表达
DOI：
10.1097/wnr.0000000000000105
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发表时间：
2014-04-16
期刊：
影响因子：
1.7
通讯作者：
Peng, Bi-wen
中科院分区：
文献类型：
--
作者：
Huang, Wen-xian;Min, Jia-wei;Peng, Bi-wen
近年来，瞬时受体电位香草素1(TRPV1)通道在包括中枢神经系统(CNS)在内的多种组织中表达。然而，TRPV1在中枢神经系统中的分布仍然是一个有争议的问题。在此，我们发现利用实时荧光定量聚合酶链式反应和免疫印迹技术可以检测到TRPV1在C57BL/6小鼠的海马区和皮质中的表达。此外，TRPV1的mRNA和蛋白表达水平在脑发育过程中呈现动态变化。与最早的2周时间点相比，4周和8周时，mRNA的表达水平逐渐升高，在较晚的时间点达到峰值，16周时开始下降，但仍维持在较高水平。然而，与2周龄的小鼠相比，其他三组(4、8和16周龄的小鼠)的表达水平总体上都有所上升。这些结果表明，在中枢神经系统中可以检测到TRPV1通道的表达，并且它在出生后的发育过程中会发生变化。
Transient receptor potential vanilloid 1 (TRPV1) channel has been found to be expressed in a variety of tissues over the last few years, including the central nervous system (CNS). However, the distribution of TRPV1 in the CNS remains a controversial question. Here, we reveal that the expression of TRPV1 can be detected in the C57BL/6 mouse hippocampus and cortex using real-time PCR and western blot. Beyond that, mRNA and protein expression levels of TRPV1 show dynamic changes during brain development. Compared with the earliest timepoint examined at 2 weeks, the expression levels of mRNA progressively increased at 4 and 8 weeks, peaking at the later timepoint, then declined at 16 weeks but remained elevated. However, compared with 2-week-old mice, the expression levels of the other three groups (4-, 8-, and 16-week-old mice) increased overall. These results indicate that TRPV1 channel expression is detectable in the CNS and it varies during postnatal development.