Pneumococcal behavior and host responses during bronchopneumonia are affected differently by the ctolytic and complement-activating activities of pneumolysin
Pneumococcal behavior and host responses during bronchopneumonia are affected differently by the ctolytic and complement-activating activities of pneumolysin
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DOI：
10.1128/iai.71.4.1813-1819.2003
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发表时间：
2003-04-01
影响因子：
3.1
通讯作者：
Andrew, PW
中科院分区：
文献类型：
--
作者：
Jounblat, R;Kadioglu, A;Andrew, PW
肺炎链球菌溶血素是由肺炎链球菌临床分离株产生的多功能毒素，与肺炎球菌性支气管肺炎和败血症的发病机制密切相关。使用同基因突变株，我们研究了删除肺炎球菌溶血素的细胞毒性活性或补体激活活性对肺和血液中细菌生长、感染肺组织的组织学变化以及炎性细胞募集模式的影响。肺炎球菌溶血素的两种活性均对肺部病理学以及菌血症发作的时间有贡献。与感染野生型肺炎球菌后观察到的变化相比，感染任一突变体后肺部的组织学变化延迟。肺炎球菌溶血素的补体激活活性影响T细胞的积累，而毒素的细胞溶解活性影响中性粒细胞募集到肺组织中。
Pneumolysin, a multifunctional toxin produced by all clinical isolates of Streptococcus pneumoniae, is strongly implicated in the pathogenesis of pneumococcal bronchopneumonia and septicemia. Using isogenic mutant strains, we examined the effect of deletion of the cytotoxic activity or complement-activating activity of pneumolysin on bacterial growth in lungs and blood, histological changes in infected lung tissue, and the pattern of inflammatory cell recruitment. Both of the activities of pneumolysin contributed to the pathology in the lungs, as well as the timing of the onset of bacteremia. Histological changes in the lungs were delayed after infection with either mutant compared to the changes seen after infection with the wild-type pneumococcus. The complement-activating activity of pneumolysin affected the accumulation of T cells, whereas the toxin's cytolytic activity influenced neutrophil recruitment into lung tissue.