Interferon gamma regulation of de novo protein synthesis in human dermal fibroblasts in culture is anatomic site dependent.
Interferon gamma regulation of de novo protein synthesis in human dermal fibroblasts in culture is anatomic site dependent.
复制标题
干扰素γ对培养的人真皮成纤维细胞从头蛋白质合成的调节具有解剖部位依赖性。
DOI：
10.1111/1523-1747.ep12469828
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Higgins,PJ
中科院分区：
文献类型：
--
作者：
Smith,TJ;Higgins,PJ
下肢前侧皮肤与格雷夫斯皮肤病相关的倾向促使我们在该区域培养成纤维细胞中检测特异性蛋白质合成和对干扰素γ的反应。取同一三位供体小腿正常皮肤和腹部皮肤的融合培养物，用[35S]蛋氨酸脉冲标记3小时，进行二维蛋白凝胶电泳和荧光成像。利用计算机驱动程序绘制蛋白质斑点，并分析可分辨斑点的相对密度。两个解剖部位的成纤维细胞显示出不同的新蛋白合成模式。在任意选择用于分析的157个丰富位点中，31%的位点之间的表达水平存在显著差异。许多蛋白质似乎只在两个解剖部位之一的培养物中表达。干扰素γ (100 U/ml)在培养基中存在48小时，以特定位点的方式影响许多蛋白质的丰度。其中，纤溶酶原激活物抑制剂i型在腿部培养物中被诱导3 - 5次，而这种多肽在腹部成纤维细胞中被下调。在干扰素处理过的两个部位的培养物中，至少50次诱导出54-kD蛋白。似乎来自被膜不同区域的成纤维细胞在其蛋白质合成程序和对细胞因子的反应方面本质上是不同的。
The propensity of the skin of the lower anterior leg to be involved in Graves' dermopathy prompted an examination of the specific protein synthesis and response to interferon gamma in cultured fibroblasts from this area. Confluent cultures from normal skin of the lower leg and from the abdomen of the same three donors were pulse labeled with [35S]methionine for 3 h and subjected to two-dimensional protein gel electrophoresis and fluorography. Protein spots were mapped using a computer-driven program and the relative densities of the resolvable spots analyzed. Fibroblasts from the two anatomic sites display distinct patterns ofde novoprotein synthesis. Of the 157 abundant spots arbitrarily chosen for analysis, 31% varied substantially in levels of expression between the sites. A number of proteins appear to be expressed only in cultures derived from one of the two anatomic sites. Interferon gamma (100 U/ml) present in the culture medium for 48 h influenced the abundance of a number of proteins in a site-specific manner. Among them, plasminogen activator inhibitor type-I was induced three to five times in the leg cultures, whereas this same polypeptide was down-regulated in abdominal fibroblasts. A 54-kD protein was induced in interferon-treated cultures from both sites at least 50 times. It appears that fibroblasts from different regions of the integument are intrinsically distinct in terms of both their protein synthetic programs and their responses to cytokines.