Equine T lymphocytes express MHC class II antigens.
Equine T lymphocytes express MHC class II antigens.
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马 T 淋巴细胞表达 MHC II 类抗原。
DOI：
10.1111/j.1744-313x.1986.tb01119.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Antczak,DF
中科院分区：
文献类型：
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作者：
Crepaldi,T;Crump,A;Newman,M;Ferrone,S;Antczak,DF
六种抗HLA II类小鼠单克隆抗体（mab）与一种针对马淋巴细胞的大鼠单克隆抗体联合使用，以确定马体内的II类主要组织相容性复合体（MHC）分子。通过ELISA检测和补体依赖性淋巴细胞毒性检测，发现6种抗HLA II类抗体中的5种和大鼠抗马单克隆抗体与外周血淋巴细胞的反应率很高。流式细胞术显示外周血淋巴细胞的抗原密度是可变的，并且有明确的证据表明淋巴细胞不携带可检测到的表面免疫球蛋白。没有一种抗体与马的血小板发生反应。单抗免疫沉淀抗原复合物m， 29,000 - 33,000从马淋巴细胞。马的MHCⅱ类抗原的分布似乎与人不同，但与狗的相似，因为MHCⅱ类抗原在静止的外周血淋巴细胞上表达，而这些淋巴细胞缺乏膜结合免疫球蛋白。MHC II类抗原在淋巴细胞亚群上的分布及其在免疫现象中的作用之间的相关性可能有助于我们了解这些分子的功能特性。
Six anti‐HLA class II mouse monoclonal antibodies (mAbs) were used in conjunction with a rat monoclonal antibody raised against horse lymphocytes to define class II major histocompatibility complex (MHC) molecules in the horse. By utilizing an ELISA assay and complement dependent lymphocytotoxicity assay, five out of the six anti‐HLA class II antibodies and the rat anti‐horse monoclonal antibody were found to react with a high percentage of peripheral blood lymphocytes. Flow cytometry demonstrated a variable antigen density on peripheral blood lymphocytes and clear evidence for expression by lymphocytes that carried no detectable surface immunoglobulin. None of the antibodies reacted with equine platelets. The mAbs immunoprecipitated an antigenic complex ofM, 29,000‐33,000 from horse lymphocytes. It appears that the distribution of MHC class II antigens in the horse is different from that in man but is similar to that in the dog, since MHC class II antigens are expressed on resting peripheral blood lymphocytes which lack membrane‐bound immunoglobulins. Correlations between the distribution of MHC class II antigens on lymphocyte subpopulations and their role in immunological phenomena may contribute to our understanding of the functional properties of these molecules.