Gfap transgenic medaka as a novel reporter line for neural stem cells
Gfap transgenic medaka as a novel reporter line for neural stem cells
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Gfap 转基因青鳉作为神经干细胞的新型报告系
DOI：
10.1016/j.gene.2022.146213
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发表时间：
2022
期刊：
影响因子：
3.5
通讯作者：
Deguchi Tomonori
中科院分区：
文献类型：
--
作者：
Shimizu Yuki;Kawasaki Takashi;Deguchi Tomonori
放射状胶质细胞(RGC)在中枢神经系统(CNS)的发育、维持和修复中起着至关重要的作用。然而，RGCs的一条特定的记者专线在青竹是有限的。胶质纤维酸性蛋白(GFAP)在包括脑和脊髓在内的硬骨中枢神经系统中含量丰富，可能是青竹中枢神经系统RGCs的候选标记物。我们获得了一种转基因青竹，其中增强型绿色荧光蛋白(EGFP)的表达受到可能的Medakagfap调控元件的调控。我们通过透明青色品系的透明体内观察到了活青竹幼虫和幼虫中枢神经系统中绿色荧光蛋白的表达。青蛙幼年和成年TG(GFAP：EGFP)的组织学分析表明，EGFP在端脑、视顶盖、视网膜和脊髓的GFAP阳性细胞中表达。我们进一步在视顶盖和视网膜中发现了另一个表达绿色荧光蛋白的细胞。根据这些EGFP阳性细胞的分布情况，这些细胞可能是神经上皮样干细胞。我们的结论是，该报告系在研究青竹中枢神经系统发育和再生过程中神经干细胞的功能方面有价值，可以可视化两种类型的神经干细胞：视网膜节细胞和神经上皮样干细胞。
Radial glial cells (RGCs) play an essential role in developing, maintaining, and repairing the central nervous system (CNS). However, a specific reporter line of RGCs is limited in medaka. Glial fibrillary acid protein (GFAP) is abundant in teleost CNS, including the brain and spinal cord, and is a possible candidate for a marker for RGCs in medaka CNS. We generated a transgenic medaka in which enhanced green fluorescent protein (EGFP) expression is regulated under putative medakagfapregulatory elements. We observed EGFP expression in the CNS of live larval and juvenile medaka through the transparent body of the See-through medaka strain. Histological analysis for juvenile and adult Tg(gfap:EGFP) medaka showed that EGFP was expressed in GFAP-positive cells in the telencephalon, optic tectum, retina, and spinal cord. We further found another EGFP expressing cells in the optic tectum and retina. These cells are possibly neuroepithelial-like stem cells, deducing from the distribution of these EGFP-positive cells. We concluded that this reporter line would be valuable in the investigation of neural stem cell function during the development and regeneration of medaka CNS visualizing two types of neural stem cells, RGCs and neuroepithelial-like stem cells.