Amino acid sequence of nitrite reductase: a copper protein from Achromobacter cycloclastes.
Amino acid sequence of nitrite reductase: a copper protein from Achromobacter cycloclastes.
复制标题
亚硝酸还原酶的氨基酸序列：来自环碎无色杆菌的铜蛋白。
DOI：
10.1021/bi00243a020
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
LeGall,J
中科院分区：
文献类型：
--
作者：
Fenderson,FF;Kumar,S;Adman,ET;Liu,MY;Payne,WJ;LeGall,J
材料和方法按照之前的描述（Liu et al., 1986）从无色杆菌中制备亚硝酸盐还原酶，然后还原，如Takio et al.（1983）所描述的s -羧甲基化或s -吡啶乙基化（Andrews & Dixon, 1987）。劈理的赖氨酸残基是通过孵化亚硝酸还原酶与无色菌蛋白酶我(t .正树博士的礼物、茨城、日本(Masaki et al ., 1981)]在50 mM Tris-HCl和2 M尿素,pH值9.0,24 h在37 C.Cleavage谷氨酸残基进行金黄色葡萄球菌的V8蛋白酶在0.1 M NH4HC03和2 M尿素,pH值8日18 h在37 c .溴化氰(2毫克)添加到蛋白质(25 nmol)在72%甲酸在黑暗中在室温下15 h。Asn-Gly键
Materials and Methods Nitrite reductase from Achromobacter cycloclastes was prepared as described previously (Liu et al., 1986), then re-duced, and either S-carboxymethylated as described by Takio et al.(1983) or S-pyridylethylated (Andrews & Dixon, 1987). Cleavage at lysine residues was accomplished by incubation of nitrite reductase with Achromobacter protease I [a gift of Dr. T. Masaki, Ibaraki, Japan (Masaki et al., 1981)] in 50 mM Tris-HCl and 2 M urea, pH 9.0, for 24 h at 37 C.Cleavage at glutamic acid residues was performed with the Staphylococcus aureus V8 protease in 0.1 M NH4HC03 and 2 M urea, pH 8, for 18 h at 37 C. Cyanogen bromide (2 mg) was added to the protein (25 nmol) in 72% formic acid for 15 h at room temperature in the dark. Asn-Gly bonds were