Apple WRKY transcription factor MdWRKY56 positively modulates drought stress tolerance
Apple WRKY transcription factor MdWRKY56 positively modulates drought stress tolerance
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DOI：
10.1016/j.envexpbot.2023.105400
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发表时间：
2023-06-01
影响因子：
5.7
通讯作者：
Ma，Fengwang
中科院分区：
文献类型：
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作者：
Duan，Dingyue;Yi，Ran;Ma，Fengwang
干旱严重威胁着农作物的生长发育和产量。WRKY转录因子（TF）在非生物胁迫反应中发挥重要作用。然而，木本植物苹果（Malus arctica）中WRKY转录因子在耐旱调控方面的研究还很有限。本研究旨在对苹果Ⅱ c群WRKY基因MdWRKY 56进行功能鉴定。干旱胁迫下MdWRKY 56的转录丰度显著上调。亚细胞定位分析显示MdWRKY 56蛋白定位于细胞核。转录激活实验证明MdWRKY 56在酵母中具有转录活性，其N-末端在该活性中起决定性作用。利用MdWRKY 56转基因拟南芥、苹果愈伤组织和苹果植株进行的功能分析表明，MdWRKY 56 TF在调节渗透胁迫和干旱胁迫耐受性方面发挥了积极作用，MdWRKY 56过表达的转基因材料表现出较低的电解质渗漏、丙二醛（MDA）含量和活性氧（ROS）积累，以及较高的鲜重、脯氨酸含量和抗氧化酶活性。综上所述，MdWRKY 56是苹果耐旱性的重要正向调节因子。
Drought seriously threatens the growth, development, and yield of crops. WRKY transcription factors (TFs) play important roles in abiotic stress responses. However, the study of WRKY TFs in woody plant apple (Malus domestica) in terms of drought tolerance regulation is limited. This study aimed to functionally characterize an apple group IIc WRKY geneMdWRKY56. The transcription abundance ofMdWRKY56was significantly upregulated under drought stress. The subcellular localization analysis revealed that the MdWRKY56 protein localized to the nucleus. Transcriptional activation experiments proved that MdWRKY56 had transcriptional activity in yeast, and its N-terminal played a decisive role in this activity. The functional analysis usingMdWRKY56transgenicArabidopsis, apple calli, and apple plants indicated that the MdWRKY56 TF functioned positively in the modulation of osmotic and drought stress tolerance, with theMdWRKY56-overexpressedtransgenic materials showing lower electrolyte leakage, malondialdehyde (MDA) content, and reactive oxygen species (ROS) accumulation, and higher fresh weight, proline content, and antioxidant enzyme activities. In conclusion, our findings indicated that MdWRKY56 served as an important positive regulator of drought stress tolerance in apple.