Quantitation of precipitating antibodies to certain soluble nuclear antigens in SLE.
Quantitation of precipitating antibodies to certain soluble nuclear antigens in SLE.
复制标题
SLE 中某些可溶性核抗原的沉淀抗体的定量。
作者：
P. Maddison;M. Reichlin
在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶中的电泳用于分离和定量洗涤的免疫沉淀物的组分。血清来自已知具有可溶性核核糖核蛋白（RNP）或可溶性核非核酸蛋白（Sm）抗体的系统性红斑狼疮患者。主要为IgG的抗体量在患者血清的0.2至8 mg/ml范围内，并且在某些情况下占总血清IgG的20%以上。结果表明，一些患者通过产生大量的特异性抗体来对疾病作出反应，并且这些抗体可以显著促进高丙种球蛋白血症。
Electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate was used to separate and quantitate the components of a washed immune precipitate. Serum was from patients with systemic lupus erythematosus known to have antibodies to soluble nuclear ribonucleoprotein (RNP) or to a soluble nuclear non-nucleic acid protein (Sm). Amounts of antibody that was predominantly IgG ranged from 0.2 to 8 mg/ml of patients' serum, and in some cases accounted for over 20% of the total serum IgG. Results demonstrate that some patients respond to the disease by producing large amounts of a specific antibody, and that these antibodies can contribute significantly to hypergammaglobulinemia.