EXTRACTION OF TRITON-X-100 AND ITS DETERMINATION IN VIRUS-INACTIVATED HUMAN PLASMA BY THE SOLVENT DETERGENT METHOD
EXTRACTION OF TRITON-X-100 AND ITS DETERMINATION IN VIRUS-INACTIVATED HUMAN PLASMA BY THE SOLVENT DETERGENT METHOD
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DOI：
10.1016/0021-9673(94)80038-3
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发表时间：
1994-01-14
影响因子：
4.1
通讯作者：
JOSIC, D
中科院分区：
文献类型：
--
作者：
STRANCAR, A;RASPOR, P;JOSIC, D
对于新鲜冷冻和冻干人血浆生产过程中的脂包膜病毒灭活，采用了溶剂-去污剂法。在该工艺中，通过用蓖麻油萃取除去溶剂磷酸三正丁酯。通过使用反相载体的固相萃取进行非离子去污剂Triton X-100的去除。为此，测试了不同的聚合物基和二氧化硅基载体。Triton X-100的最高容量是用C-18硅胶实现的。这些支持物可以结合每ml支持物超过0.1 ml的Triton X-100。没有一种蛋白质，凝血因子与支持物结合，因此它们通过柱，它们的生物活性几乎不受影响。对生产过程中洗涤剂的测定进行了研究。介绍了直接进样的专用色谱柱的应用。这是一种在等度条件下通过反相色谱法快速测定人血浆中Triton X-100的简单方法。使用这里开发的方法，小于1.0 ppm的Triton X-100可以在不到12分钟内检测到，无需任何样品预处理。
For inactivation of lipid-enveloped viruses during the production of fresh frozen and lyophilized human plasma, the solvent-detergent method was applied. In this process, the solvent tri-n-butyl phosphate is removed by extraction with castor oil. The removal of the non-ionic detergent Triton X-100 is performed by solid-phase extraction using reversed-phase supports. For this purpose, different polymer- and silica-based supports were tested. The highest capacity for Triton X-100 was achieved with C-18 silica gels. These supports can bind more than 0.1 ml of Triton X-100 per ml of support. None of the proteins, e.g., clotting factors, bind to the support and therefore they pass through the column and their biological activity is hardly affected. The determination of detergent during the production process was also studied. The application of special columns allowing direct sample injection was introduced. This is a simple method for the rapid in-process determination of Triton X-100 in human plasma by reversed-phase chromatography under isocratic conditions. Using the method developed here, less than 1.0 ppm of Triton X-100 can be detected in less than 12 min without any sample pretreatment.