Metabolome and microbiome alterations in tongue coating of gastric precancerous lesion patients
Metabolome and microbiome alterations in tongue coating of gastric precancerous lesion patients
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胃癌前病变患者舌苔代谢组和微生物组的变化
DOI：
10.1080/17474124.2021.1850259
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发表时间：
2020-12-05
影响因子：
3.9
通讯作者：
Wang, Yiqin
中科院分区：
文献类型：
--
作者：
Hao, Yiming;Zhang, Renling;Wang, Yiqin
摘要目的：本文旨在通过分析胃癌前病变（GPL）患者舌苔中的代谢物和微生物，提供对病理转变的机制见解。方法：采用GC-TOF-MS和UHPLC-QE-MS代谢组学，结合16S rRNA微生物组技术，探讨GPL患者舌苔中代谢物和微生物的变化。结果：与15个对照相比，60个GPL病例中发现133个代谢物存在差异表达，其中可分为10类。其中，鉴定出的差异表达代谢物大部分是脂质或类脂质分子。这些代谢物涉及6个代谢途径，包括甘氨酸、丝氨酸和苏氨酸代谢、精氨酸和脯氨酸代谢、鞘脂代谢、缬氨酸、亮氨酸和异亮氨酸降解、花生四烯酸代谢和酪氨酸代谢。与对照组相比，GPL 组中异普氏菌属、单杆菌属、罗氏菌属、艾肯氏菌属和聚合杆菌属的相对丰度显着增加，并且分别与脂质和类脂分子、有机氮化合物、有机氧化合物、苯丙素和聚酮化合物以及有机杂环化合物相关。结论：与健康人相比，GPL患者舌苔代谢物的变化主要表现为脂代谢的改变，并与局部微生物组的变化有关。
ABSTRACT Objective: This paper seeks to provide mechanistic insight into the pathological transition through the analysis of metabolites and microorganisms in the tongue coating of gastric precancerous lesions (GPL) patients. Methods: GC-TOF-MS and UHPLC-QE-MS metabolomics, combined with 16S rRNA microbiome techniques, were performed to explore the changes in metabolites and microorganisms in the tongue coating of GPL patients. Results: When compared with 15 controls, 133 metabolites were found to be differentially expressed in 60 GPL cases, of which could be divided into ten categories. Among them, most of the differentially expressed metabolites identified were lipids or lipid-like molecules. These metabolites were implicated in 6 metabolic pathways including glycine, serine and threonine metabolism, arginine and proline metabolism, sphingolipid metabolism, valine, leucine and isoleucine degradation, arachidonic acid metabolism, and tyrosine metabolism. The relative abundances of Alloprevotella, Solobacterium, Rothia, Eikenella, and Aggregatibacter in the GPL group increased significantly relative to the controls and were associated with lipids and lipid-like molecules, organic nitrogen compounds, organic oxygen compounds, phenylpropanoids and polyketides, and organoheterocyclic compounds, respectively. Conclusions: Compared with healthy people, the changes of tongue coating metabolites in GPL patients were mainly characterized by alterations in lipid metabolism and were associated with localized changes in the microbiome.