Microchimerism in female bone marrow and bone decades after fetal mesenchymal stem-cell trafficking in pregnancy
Microchimerism in female bone marrow and bone decades after fetal mesenchymal stem-cell trafficking in pregnancy
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DOI：
10.1016/s0140-6736(04)16631-2
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发表时间：
2004-07-10
期刊：
影响因子：
168.9
通讯作者：
Fisk, NM
中科院分区：
文献类型：
--
作者：
O'Donoghue, K;Chan, J;Fisk, NM
胎儿细胞在怀孕期间进入母体血液，并在患有自身免疫性疾病的妇女中持续存在。健康女性随后的胎母微嵌合的频率及其细胞类型尚不清楚。为了验证胎儿间充质干细胞在母体器官中持续存在的假设，我们研究了女性骨髓和肋骨。通过XY荧光原位杂交在骨髓间充质干细胞和所有男性妊娠妇女的肋骨切片中鉴定出男性细胞，但在对照组中没有（9/9 vs 0/5, p=0.0005）。我们得出的结论是，胚胎干细胞转移到母体血液中，移植到骨髓中，并在那里存活一生。这一发现对正常妊娠、增加母婴贩运的产科并发症以及移植后的移植物存活具有重要意义。
Fetal cells enter maternal blood during pregnancy and persist in women with autoimmune disease. The frequency of subsequent fetomaternal microchimerism in healthy women and its cell type is unknown. To test the hypothesis that fetal mesenchymal stem cells persist in maternal organs, we studied female bone marrow and ribs. Male cells were identified by XY fluorescence in-situ hybridisation in marrow-derived mesenchymal stem cells and in rib sections from all women with male pregnancies, but not in controls (9/9 vs 0/5, p=0.0005). We conclude that fetal stem cells transfer-red into maternal blood engraft in marrow, where they remain throughout life. This finding has implications for normal pregnancy, for obstetric complications that increase fetomaternal trafficking, and for graft survival after transplantation.