Chemical transformations in individual ultrasmall biomimetic containers
Chemical transformations in individual ultrasmall biomimetic containers
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DOI：
10.1126/science.283.5409.1892
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发表时间：
1999-03-19
期刊：
影响因子：
56.9
通讯作者：
Zare, RN
中科院分区：
文献类型：
--
作者：
Chiu, DT;Wilson, CF;Zare, RN
直径为 1 至 5 微米的单个磷脂囊泡，含有单一试剂或完整的反应系统，通过红外激光光阱或通过粘附到改性硼硅酸盐玻璃表面来固定。化学转化是通过电穿孔或电融合启动的，在每种情况下都是通过在超微电极上施加短（10微秒）强（20至50千伏/厘米）电脉冲来启动。通过远场激光荧光显微镜监测产物形成。该反应体积的超小特性导致快速扩散混合，从而可以研究快速化学动力学。该技术也非常适合研究模拟细胞膜的脂质封闭纳米环境中生物分子的反应动力学。
Individual phospholipid vesicles, 1 to 5 micrometers in diameter, containing a single reagent or a complete reaction system, were immobilized with an infrared laser optical trap or by adhesion to modified borosilicate glass surfaces. Chemical transformations were initiated either by electroporation or by electrofusion, in each case through application of a short (10-microsecond), intense (20 to 50 kilovolts per centimeter) electric pulse delivered across ultramicroelectrodes. Product formation was monitored by far-field laser fluorescence microscopy. The ultrasmall characteristic of this reaction volume led to rapid diffusional mixing that permits the study of fast chemical kinetics. This technique is also well suited for the study of reaction dynamics of biological molecules within lipid-enclosed nanoenvironments that mimic cell membranes.