Microarray-based gene profiling analysis of Muller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish.
Microarray-based gene profiling analysis of Muller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish.
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DOI：
10.1007/978-1-61779-848-1_18
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Raymond, Pamela A
中科院分区：
文献类型：
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作者：
Qin, Zhao;Raymond, Pamela A
基于微阵列的基因谱分析已经成为在全基因组范围内测量基因表达变化的重要技术。最近，细胞特异性微阵列已被报道用于研究几种模式生物中特定细胞类型的基因表达变化。在这里，我们描述了一个协议，以制备RNA样品的微阵列分析分离的穆勒神经胶质细胞衍生的视网膜干细胞从光损伤的成年斑马鱼表达的荧光标记物在穆勒细胞使用酶视网膜解离，然后通过荧光激活细胞分选（FACS）。
Microarray-based gene profiling has become an important technique to measure changes in gene expression on a genome-wide scale. Recently, cell-specific microarrays have been reported to study changes in gene expression of a particular cell type in several model organisms. Here, we describe a protocol to prepare RNA samples for microarray analysis of isolated Muller glia-derived retinal stem cells from light-damaged adult zebrafish expressing a fluorescent marker in Muller cells using enzymatic retinal dissociation followed by fluorescence-activated cell sorting (FACS).