SPECIFIC PROTEIN-METABOLISM IN IDENTIFIABLE NEURONS OF APLYSIA-CALIFORNICA
SPECIFIC PROTEIN-METABOLISM IN IDENTIFIABLE NEURONS OF APLYSIA-CALIFORNICA
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DOI：
10.1002/neu.480050306
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发表时间：
1974-01-01
期刊：
影响因子：
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通讯作者：
WOLLBERG, Z
中科院分区：
文献类型：
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作者：
GAINER, H;WOLLBERG, Z
The patterns of protein metabolism of identified cholinergic (R2, L10, and L11) and neurosecretory (R3–13, R14, and Bag cells) cells were analyzed with the use of SDS‐polyacrylamide gel electrophoresis and isoelectric focusing. L11was unique amongst the three cholinergic neurons in its relatively high rate of incorporation of labeled leucine into proteins at 12,000 daltons. All the neurosecretory cells incorporated relatively large quantities of3H‐leucine into small proteins (<12,000 daltons), which were concentrated in putative neurosecretory granule fractions of these cells. R3–13and R14synthesized a distinct basic protein which appeared specifically in the branchial nerve which contains the axons of these cells. The combination of these two analytical electrophoretic techniques allowed for the characterization of protein specificity in single nerve cells.
