Differential expression of mucins in Middle Eastern patients with colorectal cancer.
Differential expression of mucins in Middle Eastern patients with colorectal cancer.
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DOI：
10.3892/ol.2016.4672
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发表时间：
2016-07
期刊：
影响因子：
2.9
通讯作者：
Ahmad R
中科院分区：
文献类型：
--
作者：
Al-Khayal K;Abdulla M;Al-Obaid O;Zubaidi A;Vaali-Mohammed MA;Alsheikh A;Ahmad R
粘蛋白过度表达与结直肠癌的发生和发展有关。然而，关于结直肠癌中粘蛋白表达的预后重要性的数据是不一致的。由于缺乏粘蛋白表达的数据和沙特阿拉伯CRC发病率的增加，本研究的目的是分析该种族CRC患者的粘蛋白表达谱。本研究包括22例接受CRC手术的患者。对结直肠癌组织及癌旁正常组织进行组织学及免疫组化染色。从肿瘤和正常邻近样品制备组织芯片，以使用免疫组织化学研究粘蛋白表达谱。用粘蛋白1（MUC 1）、粘蛋白2（MUC 2）和粘蛋白5AC（MUC 5AC）抗体对福尔马林固定的石蜡包埋的人结直肠癌组织进行免疫染色。粘蛋白表达和组织病理学变量之间的关联进行了评估。目前的研究表明，MUC 1在早期（I期和II期; P=0.0016）和晚期（III期和IV期; P<0.0001）CRC组织中的表达高于正常癌旁组织。然而，与正常和邻近组织相比，在早期和晚期CRC组织中观察到MUC 2表达下调。此外，观察到血清MUC 1水平在早期和晚期CRC中升高。目前的研究结果表明，MUC 1的表达在早期和晚期大肠癌组织中显着较高，MUC 2在大肠癌组织中下调与正常的相邻组织相比，和血清MUC 1蛋白在大肠癌患者显着高于对照血清。总之，在结直肠肿瘤发生的MUC 1和MUC 2的表达模式是改变沙特阿拉伯CRC患者与正常相比。MUC 1的高表达可以作为CRC肿瘤不同阶段的独立生物标志物，这将有助于CRC的早期检测。
Mucin overexpression has been implicated in the tumorigenesis and progression of colorectal carcinoma (CRC). However, data obtained on the prognostic importance of mucin expression in CRC is inconsistent. Due to lack of data on mucin expression and the increase in CRC incidence in Saudi Arabia, the aim of the present study was to analyze the mucin expression profile in patients with CRC in this ethnic group. The present study consisted of 22 patients that underwent surgery for CRC. Histopathological and immunohistochemical staining was performed on CRC tumor and adjacent normal tissues. A tissue microarray was prepared from the tumor and normal adjacent samples to investigate the mucin expression profile using immunohistochemistry. Formalin-fixed paraffin-embedded human colorectal cancer tissues were immunostained with mucin 1 (MUC1), mucin 2 (MUC2) and mucin 5AC (MUC5AC) antibodies. Associations between mucin expression and histopathological variables were evaluated. The present study indicated that MUC1 was highly expressed in early (stage I and II; P=0.0016) and late (stage III and IV; P<0.0001) stage CRC tissues compared to normal adjacent tissues. However, MUC2 expression was observed to be downregulated in early and late stage CRC tissues compared to normal and adjacent tissues. Furthermore, serum MUC1 levels were observed to be increased in early and late stage CRC. The present findings indicate that MUC1 expression was significantly higher in early and late stage CRC tissues and MUC2 was downregulated in CRC tissues compared with normal adjacent tissues, and serum MUC1 protein was significantly higher in CRC patients compared to control serum. In conclusion, during colorectal tumorigenesis the pattern of MUC1 and MUC2 expression is altered in Saudi Arabian patients with CRC compared with normal. A higher expression of MUC1 may be used as an independent biomarker in various stages of CRC tumors, which would aid in the early detection of CRC.