Determination of catechin and glutathione using copper aspartate nanofibers with multiple enzyme-like activities
Determination of catechin and glutathione using copper aspartate nanofibers with multiple enzyme-like activities
复制标题
使用具有多种酶样活性的天冬氨酸铜纳米纤维测定儿茶素和谷胱甘肽
DOI：
10.1007/s00604-021-05160-x
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发表时间：
2022-02-01
影响因子：
5.7
通讯作者：
Li, Yongxin
中科院分区：
文献类型：
--
作者：
Lei, Lulu;Song, Donghui;Li, Yongxin
Copper aspartate nanofibers were facilely prepared based on aspartic acid and copper (CuAsp nanofibers). It is found that the prepared CuAsp nanofibers have catalytic activities of five enzymes, including peroxidase, laccase, catalase, ascorbate oxidase, and superoxide dismutase mimetic activities. The kinetic and catalytic properties of CuAsp nanofibers were systematically investigated, showing their high catalytic activity, excellent stability, and reusability. The laccase mimetic activity of nanofibers could be used to detect catechin in the range 20-1200 mu M with a detection limit of 5.88 mu M. In addition, a sensing platform for glutathione with a detection limit of 0.25 mu M and a detection range of 1-50 mu M was established based on CuAsp nanofibers which have the peroxidase-mimicking activity. The sensor had good selectivity and could detect glutathione in actual samples of human serum. Therefore, CuAsp nanofibers with multi-enzyme activity have broad application prospects such as biosensing, environmental management, and disease diagnosis.
