The activation of adenylate cyclase
The activation of adenylate cyclase
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腺苷酸环化酶的激活
DOI：
10.1007/bf01738682
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发表时间：
1973
影响因子：
4.3
通讯作者：
A. Constantopoulos
中科院分区：
文献类型：
--
作者：
V. Najjar;A. Constantopoulos
腺苷酸环化酶的激活的两个假设的机制。两者都需要酶以抑制的磷酸化形式和活化的去磷酸化形式存在。分别用氟化物或前列腺素（PGE 1）活化多形粒细胞（PMN）或血小板的膜制剂后，比活性如预期的那样增加。然而，当这些制剂在磷酸化系统中孵育时，活性降低。用氟或PGE 1再处理可使这种活性增加许多倍。氟处理32P标记的PMN粒细胞膜可产生无机磷酸盐。同样，PGE1处理标记的血小板膜也产生无机磷酸盐。
Two postulated mechanisms for the activation of adenylate cyclase are presented. Both require that the enzyme exist in an inhibited phosphorylated form and an activated dephosphorylated form.Following activation of membrane preparations of polymorphonuclear granulocytes (PMN) or blood platelets with fluoride or prostaglandin (PGE1) respectively, the specific activity is increased as expected. However, when such preparations are incubated in a phosphorylating system, the activity decreases. This activity can subsequently be increased many fold by retreatment with fluoride or PGE1.Fluoride treatment of32P labeled membranes of PMN granulocytes yields inorganic phosphate. Similarly, PGE1treatment of labeled platelet membranes also yields inorganic phosphate.