Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix.
Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix.
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DOI：
10.1084/jem.97.5.695
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发表时间：
1953-05
期刊：
影响因子：
--
通讯作者：
GEY GO
中科院分区：
文献类型：
--
作者：
SCHERER WF;SYVERTON JT;GEY GO
体外培养的人类上皮癌细胞已被证明支持所有三种类型的脊髓灰质炎病毒的增殖。这些细胞（HeLa细胞株）自1951年2月从宫颈表皮样癌中获得以来一直保持在体外。随着病毒的繁殖，它在12到96小时内导致癌细胞的变性和破坏。向培养物中加入同型抗体可阻止病毒的特异性破坏作用，而加入异型抗体则不能。描述了用HeLa细胞株的悬浮液制备大量重复培养物的方法。悬浮液中的细胞容易通过在血细胞计数器中直接计数来定量。维持细胞活力的合成溶液用于病毒增殖。实验结果证明了HeLa细胞株用于（a）脊髓灰质炎病毒的定量，（B）脊髓灰质炎抗体的测量，和（c）病毒的产生的有用性。
The cells of a human epithelial cancer cultivated en masse have been shown to support the multiplication of all three types of poliomyelitis virus. These cells (strain HeLa of Gey) have been maintained in vitro since their derivation from an epidermoid carcinoma of the cervix in February, 1951. As the virus multiplied it caused in from 12 to 96 hours degeneration and destruction of the cancer cells. The specific destructive effect of the virus was prevented by adding homotypic antibody to the cultures but not by adding heterotypic antibodies. Methods for the preparation of large numbers of replicate cultures with suspensions of strain HeLa cells were described. The cells in suspension were readily quantitated by direct counts in a hemocytometer. A synthetic solution that maintains cellular viability was employed for viral propagation. The experimental results demonstrate the usefulness of strain HeLa cells for (a) the quantitation of poliomyelitis virus, (b) the measurement of poliomyelitis antibodies, and (c) the production of virus.