Analysis of the product streams obtained on butanosolv pretreatment of draff
Analysis of the product streams obtained on butanosolv pretreatment of draff
复制标题
DOI：
10.1016/j.biombioe.2020.105680
复制
发表时间：
2020-10-01
影响因子：
6
通讯作者：
Westwood, Nicholas J.
中科院分区：
文献类型：
--
作者：
Foltanyi, Flora;Hawkins, Julie E.;Westwood, Nicholas J.
有效利用食品和饮料行业生物质产生的废物流对于实现循环经济非常重要。在这项工作中，基于 1-丁醇（丁醇溶剂）的预处理用于分馏啤酒和蒸馏工业的副产品残渣，产生固体纸浆、半纤维素衍生馏分和假木质素。果肉富含葡聚糖，并且在酶水解实验中相对于起始生物质显示出 4 倍的改善。纸浆可以在 ABE 工艺中发酵，产生 32 克/100 克溶剂。通过 2D HSQC NMR 分析半纤维素衍生组分，发现其主要含有丁氧基化单糖的混合物。 Cellic (R) CTec3 中存在的水解酶用于选择性水解葡萄糖和木糖衍生的丁基 β-吡喃糖单体。或者，使用 TFA/H2O 实现两种端基异构体的非选择性水解，产生用于发酵的天然糖并回收 1-丁醇。还获得了假木质素的详细表征。
The efficient use of biomass-derived waste streams from the food and drink industry is very important for achieving a circular economy. In this work, a pretreatment based on 1-butanol (butanosolv) was used to fractionate draff, a by-product from the brewing and distilling industries, leading to a solid pulp, a hemicellulose derived-fraction and a pseudo lignin. The pulp was enriched in glucans and showed a 4-fold improvement in enzymatic hydrolysis experiments relative to the starting biomass. The pulp could be fermented in an ABE process producing 32g/100g of solvents. The hemicellulose-derived fraction was analysed by 2D HSQC NMR and found to contain a mixture of predominantly butoxylated monosaccharides. The hydrolase enzymes present in Cellic (R) CTec3 were used to hydrolyse selectively the glucose and xylose derived butyl beta-pyranose monomers. Alternatively, non-selective hydrolysis of both anomers was achieved using TFA/H2O giving native sugars for fermentation and recovered 1-butanol. A detailed characterization of the pseudo lignin was also achieved.