Gradient, Contact-Free Volume Transfers Minimize Compound Loss in Dose-Response Experiments
Gradient, Contact-Free Volume Transfers Minimize Compound Loss in Dose-Response Experiments
复制标题
DOI：
10.1177/1087057109351027
复制
发表时间：
2010-01-01
影响因子：
--
通讯作者：
Ellson, Richard
中科院分区：
文献类型：
--
作者：
Harris, David;Olechno, Joe;Ellson, Richard
通过消除化合物的水溶液系列稀释，可以构建更准确的剂量-反应曲线。使用水性稀释剂的传统连续稀释可导致化合物库的显著百分比的剂量响应值的误差高达4个数量级。当使用DMSO作为稀释剂时，误差减小但没有消除。作者使用声学液滴喷射（ADE）来转移不同体积的模型库化合物，直接在接收器测定板中产生浓度梯度系列。因此，避免或最小化与化合物处理相关的样品损失和污染，特别是在水溶性较低的化合物的情况下。ADE特别适用于分析小型化，但梯度体积分配并不限于小型化应用。（Journal of Biomolecular Screening 2010：86-94）
More accurate dose-response curves can be constructed by eliminating aqueous serial dilution of compounds. Traditional serial dilutions that use aqueous diluents can result in errors in dose-response values of up to 4 orders of magnitude for a significant percentage of a compound library. When DMSO is used as the diluent, the errors are reduced but not eliminated. The authors use acoustic drop ejection (ADE) to transfer different volumes of model library compounds, directly creating a concentration gradient series in the receiver assay plate. Sample losses and contamination associated with compound handling are therefore avoided or minimized, particularly in the case of less water-soluble compounds. ADE is particularly well suited for assay miniaturization, but gradient volume dispensing is not limited to miniaturized applications. (Journal of Biomolecular Screening 2010: 86-94)