Chrysotile fiber is a strong mutagen in mammalian cells.
Chrysotile fiber is a strong mutagen in mammalian cells.
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DOI：
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发表时间：
1992-11
期刊：
影响因子：
11.2
通讯作者：
Tom K. Hei;C. Piao;Zhu Y. He;D. Vannais;C. Waldren
中科院分区：
文献类型：
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作者：
Tom K. Hei;C. Piao;Zhu Y. He;D. Vannais;C. Waldren
虽然温石棉是一种经证实的人类致癌物，但若干研究得出结论认为，这些纤维对培养的哺乳动物细胞没有致突变性。另一方面，我们在这里表明，当使用检测基因内和多位点突变的AL细胞系统进行测试时，温石棉确实具有致突变性，其强度与γ射线相当。Southern分析的诱导突变体表明，大多数包含大的删除范围从几千到几百万个碱基对的大小。我们的研究结果表明，虽然温石棉在体内的耐久性可能不如青石棉和铁石棉等闪石纤维，但它可以有效地造成癌症过程中涉及的遗传损伤。
Although chrysotile asbestos is a proven human carcinogen, several studies have concluded that these fibers are not mutagenic to cultured mammalian cells. We show here, on the other hand, that when tested using the AL cell system that detects both intragenic and multilocus mutations, chrysotile is indeed mutagenic and comparable in strength to that of gamma-rays. Southern analysis of the induced mutants shows that the majority contains large deletions ranging in size from a few thousand to several million base pairs. Results of our study demonstrate that, while chrysotile may be less durable in vivo than the amphibole fibers such as crocidolites and amosites, it can effectively create genetic damage involved in the cancer process.