Auxin Response Gene SlARF3 Plays Multiple Roles in Tomato Development and is Involved in the Formation of Epidermal Cells and Trichomes.
Auxin Response Gene SlARF3 Plays Multiple Roles in Tomato Development and is Involved in the Formation of Epidermal Cells and Trichomes.
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DOI：
10.1093/pcp/pcv136
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发表时间：
2015-11
影响因子：
4.9
通讯作者：
Xiaolan Zhang;Fang Yan;Yuwei Tang;Yujin Yuan;W. Deng;Zheng-guo Li
中科院分区：
文献类型：
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作者：
Xiaolan Zhang;Fang Yan;Yuwei Tang;Yujin Yuan;W. Deng;Zheng-guo Li
生长素反应因子 (ARF) 基因编码参与生长素信号转导的一大类蛋白质。 SlARF3 是 ARF 基因家族的成员，编码含有两个保守结构域（B3 和 ARF）且缺乏 Aux/IAA 结构域的蛋白质。表达分析表明SlARF3在毛状体中具有特别高的表达水平。原位杂交还在叶子的表皮铺装细胞中检测到了 SlARF3 转录本。利用RNA干扰(RNAi)策略研究SlARF3的生理功能。 SlARF3下调植株表皮铺装细胞密度降低，叶片I、V、VI型毛状体密度明显降低，表明SlARF3在番茄毛状体和表皮细胞形成中的重要作用。 SlARF3 下调株系中芽木质部细胞的数量也减少。此外，RNA测序（RNA-Seq）分析确定了属于14个转录因子（TF）家族的51个差异表达基因（DEG），例如MYB、bHLH、WD40和C2H2锌指。 27个DEG参与植物激素（如生长素、乙烯和赤霉素）的代谢和信号转导。这些结果表明转录因子和激素在番茄毛状体形成的生长素依赖性转录调节中的重要作用。综上所述，我们的结果表明 SlARF3 在表皮细胞和毛状体的形成中发挥着重要作用，并揭示了 ARF 在番茄发育过程中的新颖且特定的功能。
The auxin response factor (ARF) genes encode a large family of proteins involved in auxin signaling transduction. SlARF3, a member of the ARF gene family, encodes a protein containing two conserved domains, B3 and ARF, and lacking an Aux/IAA domain. Expression analysis showed that SlARF3 has a particularly high expression level in trichomes. In situ hybridization also detected the SlARF3 transcripts in epidermal pavement cells of leaves. The physiological function of SlARF3 was studied by using the RNA interference (RNAi) strategy. SlARF3-down-regulated plants exhibited decreased density of epidermal pavement cells and obviously reduced density of type I, V and VI trichomes of leaves, which indicates the important role of SlARF3 in the formation of trichomes and epidermal cells in tomato. The number of shoot xylem cells was also decreased in SlARF3-down-regulated lines. Furthermore, RNA-sequencing (RNA-Seq) analysis identified 51 differentially expressed genes (DEGs) belonging to 14 transcription factor (TF) families, such as MYB, bHLH, WD40 and C2H2 zinc finger. Twenty-seven DEGs were involved in the metabolism and signaling transduction of phytohormones, such as auxin, ethylene and gibberellin. These results indicated the important roles of the TFs and hormones in auxin-dependent transcriptional regulation of trichome formation in tomato. Taken together, our results demonstrate that SlARF3 plays an important role in the formation of epidermal cells and trichomes and reveal novel and specific functions for ARFs in tomato developmental processes.