EFFECTS OF A SERUM SPREADING FACTOR ON GROWTH AND MORPHOLOGY OF CELLS IN SERUM-FREE MEDIUM
EFFECTS OF A SERUM SPREADING FACTOR ON GROWTH AND MORPHOLOGY OF CELLS IN SERUM-FREE MEDIUM
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DOI：
10.1002/jss.400140106
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
SATO, G
中科院分区：
文献类型：
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作者：
BARNES, D;WOLFE, R;SATO, G
从人血清中部分纯化出一种热敏、胰蛋白酶敏感因子，可促进细胞 [大鼠神经胶质瘤 C6 细胞] 在无血清、补充激素的培养基中生长和扩散。这些制剂中的大部分蛋白质在 SDS[十二烷基硫酸钠]-聚丙烯酰胺凝胶电泳上迁移，迁移率与 60,000-90,000 之间的 MW 一致。这种扩散活性被称为血清扩散因子，刺激多种细胞类型的生长和扩散。血清扩散因子与纤连蛋白相似，因为它显示出对塑料细胞培养基质的亲和力，但根据几个标准显示其与纤连蛋白不同。该因子可能在细胞附着和扩散的研究以及细胞形状和细胞增殖的关系的研究中有用。
A heat-sensitive, trypsin-sensitive factor that promoted growth and spreading of cells [rat glioma C6 cell] in serum-free, hormone-supplemented medium was partially purified from human serum. The major portion of the proteins in these preparations migrated upon SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis with a mobility consistent with MW between 60,000-90,000. The spreading activity, which were termed serum spreading factor, stimulated growth and spreading of a wide variety of cell types. The serum spreading factor was similar to fibronectin in that it showed an affinity for the plastic cell culture substrate but was shown to be distinct from fibronectin by several criteria. This factor may prove useful in studies of cell attachment and spreading and in studies of the relationship of cell shape and cell proliferation.