A 21-aminosteroid reduces hydrogen peroxide generation by and chemiluminescence of stimulated human leukocytes.
A 21-aminosteroid reduces hydrogen peroxide generation by and chemiluminescence of stimulated human leukocytes.
复制标题
21-氨基类固醇可减少受刺激的人类白细胞的过氧化氢生成和化学发光。
作者：
M. Fisher;P. Levine;Ronald A. Cohen
白细胞募集到局灶性脑缺血区域可能通过其促进炎症的能力而导致组织损伤。一组新的药物，21-氨基类固醇，已被观察到减少中风动物模型中的神经损伤和血管源性脑水肿，通过抑制脂质过氧化。炎症反应期间白细胞产生的过氧化氢和自由基可能导致脂质过氧化和自由基介导的组织损伤的其他后果。我们评估了U 74500 A（一种21-氨基类固醇）对过氧化氢生成的影响，以及对正常人受刺激的多形核白细胞和单核细胞的化学发光的影响。U 74500 A以剂量依赖性方式显著减少多形核白细胞（p <0.001）和单核细胞（p <0.01）产生的过氧化氢。单核细胞化学发光也被显著抑制（p <0.05），但多形核白细胞相关化学发光无变化。我们的研究结果表明，U 74500 A可以降低与刺激的人白细胞相关的氧代谢产物的浓度，这种作用可以部分解释21-氨基类固醇如何减少脂质过氧化，缺血性损伤和血管源性水肿。
Leukocytes recruited to regions of focal cerebral ischemia may contribute to tissue injury by their ability to promote inflammation. A novel group of drugs, the 21-aminosteroids, have been observed to reduce neurologic damage and vasogenic cerebral edema in animal models of stroke by inhibiting lipid peroxidation. Production of hydrogen peroxide and free radicals by leukocytes during the inflammatory response may contribute to lipid peroxidation and other consequences of free radical-mediated tissue injury. We assessed the effect of U74500A, a 21-aminosteroid, on the generation of hydrogen peroxide by and on the chemiluminescence of stimulated polymorphonuclear leukocytes and monocytes from normal humans. U74500A significantly reduced the generation of hydrogen peroxide by polymorphonuclear leukocytes (p less than 0.001) and monocytes (p less than 0.01) in a dose-dependent manner. Monocyte chemiluminescence was also significantly inhibited (p less than 0.05), but polymorphonuclear leukocyte-associated chemiluminescence was unchanged. Our results indicate that U74500A can reduce the concentration of oxygen metabolites associated with stimulated human leukocytes, and this effect may explain in part how 21-aminosteroids reduce lipid peroxidation, ischemic injury, and vasogenic edema.
影响因子：
--
作者：
NELSON, CW;WEI, EP;MOSKOWITZ, MA
通讯作者：
MOSKOWITZ, MA
21-氨基类固醇可减少大鼠大脑中动脉局部缺血模型中的离子迁移和水肿。
影响因子：
8.3
作者：
Young,W;Wojak,JC;DeCrescito,V
通讯作者：
DeCrescito,V