Determination of calcium and magnesium in serum and urine by atomic absorption spectrophotometry.
Determination of calcium and magnesium in serum and urine by atomic absorption spectrophotometry.
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DOI：
10.1016/0009-8981(69)90046-1
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发表时间：
1969-02
期刊：
影响因子：
--
通讯作者：
J. Pybus
中科院分区：
文献类型：
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作者：
J. Pybus
建立了一种灵敏、准确的原子吸收光谱法测定血、尿中钙、镁的方法。锶的加入消除了磷酸盐抑制，并用0.1N高氯酸消除了蛋白质的干扰。这两种金属的准确度是通过与干灰化技术的比较而建立的，钙的准确度是通过与使用钙黄绿素终点的EDTA滴定进行比较而建立的。在盲样中，钙的技术误差为±0.13 mg/100毫升，镁的技术误差为±0.04毫克/100毫升。钙的正常范围是9.3-10.3毫克/100毫升，镁的正常范围是1.9-2.3毫克/100毫升。已经研究了尿液成分、火焰条件、可变雾化器的使用、稀释的影响和其他参数的可能干扰，并且不影响这两种元素的结果。
A sensitive and accurate method for measuring calcium and magnesium in serum and urine by atomic absorption spectrophotometry is described. Phosphate depression has been eliminated by the addition of strontium, and 0.1Nperchloric acid has been used to remove protein interference. Accuracy for both metals has been established by comparison with a dry ashing technique, and for calcium by comparison with an EDTA titration using a calcein end point. A technical error of ± 0.13 mg/100 ml for calcium and ± 0.04 mg/100 ml for magnesium has been obtained in blind duplicates. The normal range found for calcium is 9.3–10.3 mg/100 ml and 1.9–2.3 mg/100 ml for magnesium. Possible interference from urinary constituents, flame conditions, the use of a variable atomiser, effect of dilution and other parameters have been investigated and do not affect the results for either element.