Applications of Single-Cell Sequencing Technology to the Enteric Nervous System.
Applications of Single-Cell Sequencing Technology to the Enteric Nervous System.
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DOI：
10.3390/biom12030452
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发表时间：
2022-03-15
期刊：
影响因子：
5.5
通讯作者：
Goldstein AM
中科院分区：
文献类型：
--
作者：
Guyer RA;Mueller JL;Goldstein AM
随着最近的技术进步和测序成本的降低，单细胞测序模式已经变得司空见惯。这些工具允许在单细胞分辨率下分析RNA表达、DNA序列、染色质结构和细胞表面抗原。同时测量多个参数可以解析包括稀有细胞在内的群体，从而揭示器官内的细胞多样性，并允许发育组织中的谱系重建。将这些方法应用于肠神经系统已经产生了丰富的数据和生物学见解。我们回顾最近的论文应用单细胞测序工具的新生神经嵴和发展和成熟的肠神经系统。这些研究表明，肠道神经元和神经胶质细胞的显着多样性，神经元规格的建议范例，并揭示在发展过程中活跃的信号通路。随着技术的发展和多基因组技术结合两个或更多的转录组，基因组，表观遗传和蛋白质组数据变得突出，我们预计这些模式将成为ENS研究中的常见现象，并可能在诊断测试和个性化治疗中发挥作用。
With recent technical advances and diminishing sequencing costs, single-cell sequencing modalities have become commonplace. These tools permit analysis of RNA expression, DNA sequence, chromatin structure, and cell surface antigens at single-cell resolution. Simultaneous measurement of numerous parameters can resolve populations including rare cells, thus revealing cellular diversity within organs and permitting lineage reconstruction in developing tissues. Application of these methods to the enteric nervous system has yielded a wealth of data and biological insights. We review recent papers applying single-cell sequencing tools to the nascent neural crest and to the developing and mature enteric nervous system. These studies have shown significant diversity of enteric neurons and glia, suggested paradigms for neuronal specification, and revealed signaling pathways active during development. As technology evolves and multiome techniques combining two or more of transcriptomic, genomic, epigenetic, and proteomic data become prominent, we anticipate these modalities will become commonplace in ENS research and may find a role in diagnostic testing and personalized therapeutics.
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影响因子：
11.8
作者：
Elmentaite R;Ross ADB;Roberts K;James KR;Ortmann D;Gomes T;Nayak K;Tuck L;Pritchard S;Bayraktar OA;Heuschkel R;Vallier L;Teichmann SA;Zilbauer M
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作者：
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通讯作者：
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影响因子：
6.2
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通讯作者：
Pachnis, Vassilis
影响因子：
2.4
作者：
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通讯作者：
Goldstein AM