Neutralization of SARS-CoV-2 Variants of Concern Harboring Q677H.
Neutralization of SARS-CoV-2 Variants of Concern Harboring Q677H.
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DOI：
10.1128/mbio.02510-21
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发表时间：
2021-10-26
期刊：
影响因子：
6.4
通讯作者：
Liu SL
中科院分区：
文献类型：
--
作者：
Zeng C;Evans JP;Faraone JN;Qu P;Zheng YM;Saif L;Oltz EM;Lozanski G;Gumina RJ;Liu SL
SARS-CoV-2 相关变体 (VOC) 对中和抗体的敏感性主要是在关键受体结合域 (RBD) 突变（包括 E484K 和 N501Y）的背景下进行的研究。关于 SARS-CoV-2 刺突突变组合的上位效应知之甚少。我们现在研究含有非 RBD 突变 Q677H 的变体的中和敏感性，包括 B.1.525（尼日利亚分离株）和 Bluebird（美国分离株）变体。 Q677H 的中和效果是在 RBD 突变和主要 VOC 背景下确定的，包括 B.1.1.7（英国，Alpha）、B.1.351（南非，Beta）和 P1-501Y-V3（巴西，Gamma）。我们证明 Q677H 突变增加了病毒感染性和合胞体形成，并增强了对 VOC 中和的抵抗力，包括 B.1.1.7 和 P1-501Y-V3。我们的工作强调了 SARS-CoV-2 尖峰突变之间上位相互作用的重要性以及持续监测含有 Q677H 的 VOC 的必要性。
The sensitivity of SARS-CoV-2 variants of concern (VOCs) to neutralizing antibodies has largely been studied in the context of key receptor binding domain (RBD) mutations, including E484K and N501Y. Little is known about the epistatic effects of combined SARS-CoV-2 spike mutations. We now investigate the neutralization sensitivity of variants containing the non-RBD mutation Q677H, including B.1.525 (Nigerian isolate) and Bluebird (U.S. isolate) variants. The effect on neutralization of Q677H was determined in the context of the RBD mutations and in the background of major VOCs, including B.1.1.7 (United Kingdom, Alpha), B.1.351 (South Africa, Beta), and P1-501Y-V3 (Brazil, Gamma). We demonstrate that the Q677H mutation increases viral infectivity and syncytium formation, as well as enhancing resistance to neutralization for VOCs, including B.1.1.7 and P1-501Y-V3. Our work highlights the importance of epistatic interactions between SARS-CoV-2 spike mutations and the continued need to monitor Q677H-bearing VOCs.