Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier.
Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No. 1 glacier.
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DOI：
10.1099/ijs.0.64750-0
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发表时间：
2007-04
影响因子：
2.8
通讯作者：
Dechao Zhang;He-Xiang Wang;Heng-Lin Cui;Yong Yang;Hong-Can Liu;Xiuzhu Dong;P. Zhou
中科院分区：
文献类型：
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作者：
Dechao Zhang;He-Xiang Wang;Heng-Lin Cui;Yong Yang;Hong-Can Liu;Xiuzhu Dong;P. Zhou
从中国一号冰川中分离到一株耐低温、革兰氏阳性、黄色、需氧菌0549（T）。该分离物的细胞为过氧化氢酶阳性、能动、不规则的杆状。细胞壁肽聚糖的二氨基酸含量测定为2，4-二氨基丁酸。菌株0549（T）能够在4-27 ℃生长，最佳生长发生在20-22 ℃。主要脂肪酸为anteiso-C（15：0）、anteiso-C（15：1）、iso-C（16：0）和anteiso-C（17：0）。基因组DNA G+C含量为67mol%。基于16 S rRNA基因序列的系统发育分析表明，菌株0549（T）属于Cryobacterium属，与Cryobacterium psychrophilum JCM 1463（T）的同源性为96.5%。基于表型、化学分类学和系统发育学特性以及DNA-DNA相关性数据，菌株0549（T）代表了Cryobacterium属的一个新种，建议将其命名为Cryobacterium psychrotolerans。模式菌株为0549（T）（=CGMCC 1.5382（T）=JCM 13925（T））。
A novel psychrotolerant, Gram-positive, yellow-pigmented, aerobic bacterium, strain 0549(T), was isolated from the China No. 1 glacier. The cells of the isolate were catalase-positive, motile, irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain 0549(T) was able to grow at 4-27 degrees C, with optimum growth occurring at 20-22 degrees C. The major fatty acids were anteiso-C(15 : 0), anteiso-C(15 : 1), iso-C(16 : 0) and anteiso-C(17 : 0). The genomic DNA G+C content was 67 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 0549(T) belonged to the genus Cryobacterium and exhibited 96.5 % similarity to Cryobacterium psychrophilum JCM 1463(T). On the basis of the phenotypic, chemotaxonomic and phylogenetic properties and the DNA-DNA relatedness data, strain 0549(T) represents a novel species of the genus Cryobacterium, for which the name Cryobacterium psychrotolerans is proposed. The type strain is 0549(T) (=CGMCC 1.5382(T)=JCM 13925(T)).