Use of Impedance Spectroscopy for the Characterization of In-Vitro Osteoblast Cell Response in Porous Titanium Bone Implants
Use of Impedance Spectroscopy for the Characterization of In-Vitro Osteoblast Cell Response in Porous Titanium Bone Implants
复制标题
DOI：
10.3390/met10081077
复制
发表时间：
2020-08-01
期刊：
影响因子：
2.9
通讯作者：
Torres, Yadir
中科院分区：
文献类型：
--
作者：
Giner, Merce;Olmo, Alberto;Torres, Yadir
使用具有足够孔隙率（含量、尺寸和形态）的钛植入物可以解决传统钛植入物中出现的应力屏蔽限制。评估植入物上的细胞反应（成骨细胞的粘附、增殖和分化）的实验是昂贵、耗时且精细的。在这项工作中，我们建议使用阻抗谱来评估多孔钛植入物上成骨细胞的生长。成骨细胞培养在完全致密的 40 vol.% 多孔盘上，具有两种孔径范围（100-200 μm 和 355-500 μm），以研究细胞活力、增殖、分化（碱性磷酸酶活性）和细胞形态。 40 vol.%（100-200 μm）的多孔基质显示出改善的骨整合反应，在 21 天时通过碱性磷酸酶 (ALP) 实现了超过 80% 的细胞活力和更高水平的细胞分化。进一步评估了这种细胞行为，观察到细胞附着时所有研究条件下阻抗模量的增加。然而，由于其表面特性（平坦表面），全致密的阻抗水平高于多孔基材（平坦和孔壁）。表面参数对整体测量阻抗起着重要作用。阻抗对于表征不同样品类型中的细胞培养物非常有用。
The use of titanium implants with adequate porosity (content, size and morphology) could solve the stress shielding limitations that occur in conventional titanium implants. Experiments to assess the cellular response (adhesion, proliferation and differentiation of osteoblasts) on implants are expensive, time-consuming and delicate. In this work, we propose the use of impedance spectroscopy to evaluate the growth of osteoblasts on porous titanium implants. Osteoblasts cells were cultured on fully-dense and 40 vol.% porous discs with two ranges of pore size (100-200 mu m and 355-500 mu m) to study cell viability, proliferation, differentiation (Alkaline phosphatase activity) and cell morphology. The porous substrates 40 vol.% (100-200 mu m) showed improved osseointegration response as achieved more than 80% of cell viability and higher levels of Cell Differentiation by Alkaline Phosphatase (ALP) at 21 days. This cell behavior was further evaluated observing an increase in the impedance modulus for all study conditions when cells were attached. However, impedance levels were higher on fully-dense due to its surface properties (flat surface) than porous substrates (flat and pore walls). Surface parameters play an important role on the global measured impedance. Impedance is useful for characterizing cell cultures in different sample types.