NEUTROPHIL AND MONOCYTE CELL-SURFACE P150,95 HAS IC3B-RECEPTOR (CR4) ACTIVITY RESEMBLING CR3
NEUTROPHIL AND MONOCYTE CELL-SURFACE P150,95 HAS IC3B-RECEPTOR (CR4) ACTIVITY RESEMBLING CR3
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DOI：
10.1172/jci113643
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发表时间：
1988-08-01
影响因子：
15.9
通讯作者：
ROSS, GD
中科院分区：
文献类型：
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作者：
MYONES, BL;DALZELL, JG;ROSS, GD
p150,95 （CD11c）是CD18膜糖蛋白家族的第三个成员，包括CR3 （Mac-1或CD11b）和LFA-1 (CD11a)，先前的研究表明，溶解的p150,95以类似于分离的CR3的方式结合到ic3b -脂糖上。目前的研究表明，膜表面p150,95也表达iC3b受体活性，可能与先前指定为CR4的iC3b或c3dg包被红细胞（EC3bi或EC3dg）的中性粒细胞受体相同。正常中性粒细胞和巨噬细胞依赖cr4的EC3bi莲座被单克隆抗p150,95抑制，来自CD18缺乏症患者的细胞不能形成依赖cr4的EC3bi莲座。对于含有大量CR1和CR3以及少量p150,95的中性粒细胞，EC3bi主要通过CR1和CR3结合，并且证明p150,94依赖的花环需要大量固定iC3b，低离子强度缓冲液和抗体阻断CR1和CR3。相比之下，培养源性巨噬细胞表达的p150、95是单核细胞的8倍，当EC3bi携带少量固定的iC3b并在等渗缓冲液中进行检测时，EC3bi与p150、95和CR3结合。通过免疫过氧化物酶染色比较各组织中CR1、CR3和CR4的含量，发现CR4是组织巨噬细胞中含量最多的C3受体分子，提示CR4可能参与了C3活化颗粒或免疫复合物的清除。
Previous investigations of p150,95 (CD11c), the third member of the CD18 membrane glycoprotein family that includes CR3 (Mac-1 or CD11b) and LFA-1 (CD11a), had demonstrated that solubilized p150,95 bound to iC3b-agarose in a manner similar to isolated CR3. The current study showed that membrane surface p150,95 also expressed iC3b-receptor activity and was probably the same as the neutrophil receptor for iC3b- or C3dg-coated erythrocytes (EC3bi or EC3dg) that had been previously designated CR4. Normal neutrophil and macrophage CR4-dependent EC3bi rosettes were inhibited by monoclonal anti-p150,95, and cells from a patient with CD18 deficiency did not form CR4-dependent EC3bi rosettes. With neutrophils that bore large amounts of CR1 and CR3 and little p150,95, EC3bi were bound primarily via CR1 and CR3, and demonstration of p150,94-dependent rosettes required large amounts of fixed iC3b, low-ionic strength buffer, and antibody blockade of CR1 and CR3. By contrast, culture-derived macrophages expressed eight times more p150,95 than did monocytes and EC3bi were bound to both p150,95 and CR3 when EC3bi bore small amounts of fixed iC3b and assays were carried out in isotonic buffer. Comparison of the amounts of CR1, CR3, and CR4 in various tissues by immunoperoxidase staining revealed that CR4 was the most abundant C3 receptor molecule on tissue macrophages, and suggested that CR4 might be involved in clearance of C3-opsonized particles or immune complexes.