Transgenic tomato overexpressing ath-miR399d has enhanced phosphorus accumulation through increased acid phosphatase and proton secretion as well as phosphate transporters
Transgenic tomato overexpressing ath-miR399d has enhanced phosphorus accumulation through increased acid phosphatase and proton secretion as well as phosphate transporters
复制标题
过表达 ath-miR399d 的转基因番茄通过增加酸性磷酸酶和质子分泌以及磷酸盐转运蛋白来增强磷积累
DOI：
10.1007/s11104-009-0219-3
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发表时间：
2010-09-01
期刊：
影响因子：
4.9
通讯作者：
Shi, Weiming
中科院分区：
文献类型：
--
作者：
Gao, Nan;Su, Yanhua;Shi, Weiming
本研究通过在水培条件下、石英砂中、大棚蔬菜土壤培养中培养和检测过表达ath-miR 399 d的转基因番茄，旨在研究miR 399 d对磷积累、过表达ath-miR 399 d的转基因番茄芽中磷浓度、磷转运蛋白表达、根的质子分泌和酸性磷酸酶（AP酶）活性。转基因番茄叶片磷浓度显著增加，根系对磷的吸收也显著增加，表现为叶片磷浓度和LePT 1、LePT 2、LePT 4和LePT 5基因在正常磷溶液中的相对表达量。增强APase活性的转基因根和外部介质导致上级水解有机P，并增加质子挤出根导致上级溶解AlPO 4。因此，除了磷转运蛋白外，较高的AP酶活性和根部附近酸化作用的增强可能是转基因番茄吸收或吸收土壤磷的重要机制。这些结果提供了对miR 399过表达植物在芽中积累过量P的新理解。
By generating and examining transgenic tomato overexpressing ath-miR399d grown in hydroponic conditions, in quartz sand, or in a polytunnel greenhouse vegetable soil culture, this study aimed to investigate the effects of miR399d fromArabidopsison phosphorus (P) accumulation, P concentrations in transgenic tomato overexpressing ath-miR399d shoots, phosphate transporter expression, and proton secretion and acid phosphatase (APase) activity in roots. In the transgenic tomato, leaf P concentration increased significantly in an agricultural soil, and roots had higher uptake of P, as evidenced by leaf P concentrations and relative expression of the genesLePT1,LePT2,LePT4, andLePT5in normal-P solution. Enhanced APase activity in transgenic roots and the outside medium led to superior hydrolysis of organic P, and increased proton extrusion by roots led to superior dissolution of AlPO4. Thus, besides phosphate transporters, higher APase activity and strengthened acidification in the vicinity of the roots may be important mechanisms for transgenic tomato to scavenge or acquire P in soil. These results provide new understanding of miR399-overexpressing plants that accumulate excess P in shoots.