Mycobacterium tuberculosis Beijing Genotype in Russia: in Search of Informative Variable-Number Tandem-Repeat Loci
Mycobacterium tuberculosis Beijing Genotype in Russia: in Search of Informative Variable-Number Tandem-Repeat Loci
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DOI：
10.1128/jcm.00414-08
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发表时间：
2008-11-01
影响因子：
9.4
通讯作者：
Rastogi, Nalin
中科院分区：
文献类型：
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作者：
Mokrousov, Igor;Narvskaya, Olga;Rastogi, Nalin
北京基因型是结核分枝杆菌的全球传播谱系。在俄罗斯，这些菌株占当地M.结核病;它们与多药耐药性有关，并显示出更高的传播性。在这里，我们分析了传统的和新的标记，快速和简单的基因分型的北京菌株。从彼得堡巴斯德研究所的IS 6110限制性片段长度（RFLP）数据库中选择120株北京基因型菌株的代表性样本。使用新提出的格式的24个基因座和3个高变（HV）基因座（QUB-3232、VNTR-3820和VNTR-4120）对这些菌株进行可变数目串联重复（VNTR）分型。27个VNTR位点中有10个是单态的，而MIRU-26、QUB-26、QUB-3232、VNTR-3820和VNTR-4120这5个位点是最多态的（Hunter Gaston指数，> 0.5）。VNTR分型使我们能够区分两个大的IS 6110-RFLP簇，这两个簇已知在整个国家流行（簇B 0/W148和A0），并在北京基因型数据库中分别鉴定出27%和23%的菌株。B 0/W148菌株在VNTR树状图中紧密分组，并且可以通过位点MIRU 26和QUB-26的特征签名来区分。因此，这种临床上重要的IS 6110-RFLP变异体，B 0/W148，可能在M内呈现成功的克隆群。结核病北京血统，是广泛分布在俄罗斯。综上所述，IS 6110-RFLP方法和VNTR分型使用一组减少的最多态位点互补的高分辨率流行病学分型的M。在俄罗斯传播或从俄罗斯输入的北京基因型结核病菌株。
The Beijing genotype is a globally spread lineage of Mycobacterium tuberculosis. In Russia, these strains constitute half of the local population of M. tuberculosis; they are associated with multidrug resistance and show increased transmissibility. Here, we analyzed traditional and new markers for the rapid and simple genotyping of the Beijing strains. A representative sample of 120 Beijing genotype strains was selected from a local IS6110-restriction fragment length (RFLP) database at the St. Petersburg Pasteur Institute. These strains were subjected to variable-number tandem-repeat (VNTR) typing using 24 loci of a newly proposed format and three hypervariable (HV) loci (QUB-3232, VNTR-3820, and VNTR-4120). Ten of the 27 VNTR loci were monomorphic, while five loci, MIRU26, QUB-26, QUB-3232, VNTR-3820, and VNTR-4120, were the most polymorphic (Hunter Gaston index, > 0.5). VNTR typing allowed us to differentiate between two large IS6110-RFLP clusters known to be prevalent across the entire country (clusters B0/W148 and A0) and identified in 27 and 23% of strains, respectively, in the Beijing genotype database. The B0/W148 strains were grouped closely in the VNTR dendrogram and could be distinguished by a characteristic signature of the loci MIRU26 and QUB-26. Consequently, this clinically important IS6110-RFLP variant, B0/W148, likely presents a successful clonal group within the M. tuberculosis Beijing lineage that is widespread in Russia. To conclude, the IS6110-RFLP method and VNTR typing using a reduced set of the most polymorphic loci complement each other for the high-resolution epidemiological typing of the M. tuberculosis Beijing genotype strains circulating in or imported from Russia.