Functional regionalization of the teleost cerebellum analyzed in vivo
Functional regionalization of the teleost cerebellum analyzed in vivo
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DOI：
10.1073/pnas.1403105111
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发表时间：
2014-08-12
影响因子：
11.1
通讯作者：
Koester, Reinhard W.
中科院分区：
文献类型：
--
作者：
Matsui, Hideaki;Namikawa, Kazuhiko;Koester, Reinhard W.
越来越多的证据表明，小脑具有区域化的组织，这主要是由小脑传入事件轴突投射的解剖图推断出来的。病变研究和染料示踪实验也提出了类似的小脑输出区域化，但其生理目标以及这种输出区域化的功能相关性尚不清楚。理想情况下，这种功能区划应该在体内被证明是非侵入性的。我们在此通过斑马鱼浦肯野神经元输出回路的遗传编码跨神经元映射，为小脑皮层输出的区域化提供了证据。这些已识别的回路对应于自由行为的斑马鱼幼虫在小脑依赖行为表现期间独特的区域浦肯野细胞活动模式。此外，对动物行为中特定浦肯野细胞区域的光遗传学研究证实了浦肯野细胞输出的功能区域化，揭示了它们对行为控制的贡献以及它们在控制侧化行为输出中的功能。我们的发现揭示了脑区是如何通过将专门的传出回路用于不同的行为任务来实现多种功能的。
There has been accumulating evidence for a regionalized organization of the cerebellum, which was mostly deduced from anatomical mapping of axonal projections of cerebellar afferents. A likewise regionalization of the cerebellar output has been suggested from lesion studies and dye-tracer experiments, but its physiological targets as well as the functional relevance of such an output regionalization are less clear. Ideally, such functional regionalization should be proven noninvasively in vivo. We here provide evidence for such a regionalization of the output from the cerebellar cortex by genetically encoded transneuronal mapping of efferent circuits of zebrafish Purkinje neurons. These identified circuits correspond to distinct regionalized Purkinje cell activity patterns in freely behaving zebrafish larvae during the performance of cerebellar-dependent behaviors. Furthermore, optogenetic interrogation of selected Purkinje cell regions during animal behavior confirms the functional regionalization of Purkinje cell efferents and reveals their contribution to behavior control as well as their function in controlling lateralized behavioral output. Our findings reveal how brain compartments serve to fulfill a multitude of functions by dedicating specialized efferent circuits to distinct behavioral tasks.