Cannabinoid receptor CB1 mRNA is highly expressed in the rat ciliary body:: implications for the antiglaucoma properties of marihuana
Cannabinoid receptor CB1 mRNA is highly expressed in the rat ciliary body:: implications for the antiglaucoma properties of marihuana
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DOI：
10.1016/s0169-328x(98)00105-3
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发表时间：
1998-07-15
期刊：
影响因子：
--
通讯作者：
Pani, L
中科院分区：
文献类型：
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作者：
Porcella, A;Casellas, P;Pani, L
我们使用RT-PCR来测量大鼠眼内大麻素受体（CB）mRNA的相对差异，将CB 1或CB 2转录本与正常化参考基因β 2微球蛋白（β 2 m）的转录本进行比较，在睫状体中发现CB 1 mRNA水平显著较高（0.84 +/-0.05%beta2 m）比在虹膜（0.34 +/-0.04%beta2 m）、视网膜（0.07 +/-0.005%beta2 m）和脉络膜（0.06 +/-0.005%beta2 m）中检测不到CB 2 mRNA。这种表达模式支持CB 1受体在控制眼内压中的特定作用，有助于解释大麻素的抗青光眼特性。(C)1998 Elsevier Science B. V.保留所有权利。
We used RT-PCR to measure relative differences in cannabinoid receptor (CB) mRNAs in the rat eye, comparing CB1 or CB2 transcripts to that of the normalizing reference gene beta(2) microglobulin (beta(2)m) significantly higher levels of CB1 mRNA levels were found in the ciliary body (0.84 +/- 0.05% of beta(2)m) than in the iris, (0.34 +/- 0.04% of beta(2)m), retina (0.07 +/- 0.005% of beta(2)m) and choroid (0.06 +/- 0.005% of beta(2)m) CB2 mRNA was undetectable. This expression pattern supports a specific role for the CB1 receptor in controlling intraocular pressure, helping to explain the antiglaucoma property of cannabinoids. (C) 1998 Elsevier Science B.V. All rights reserved.