Structural changes in thestrial blood-labyrinth barrier of aged C57BL/6 mice.
Structural changes in thestrial blood-labyrinth barrier of aged C57BL/6 mice.
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DOI：
10.1007/s00441-015-2147-2
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发表时间：
2015-09
影响因子：
3.6
通讯作者：
Shi X
中科院分区：
文献类型：
--
作者：
Neng L;Zhang J;Yang J;Zhang F;Lopez IA;Dong M;Shi X
严格控制耳蜗血流（CoBF）和血管纹中的血迷路屏障（BLB）对于维持听力功能所需的离子、液体和能量平衡至关重要。CoBF不足和BLB完整性的破坏一直被认为是各种听力障碍的主要病因。在这项研究中，我们研究了年龄分级的C57 BL/6小鼠（1个月至21个月）的血管纹BLB的结构变化，重点是两个血液屏障辅助细胞，周细胞（PC）和血管周围驻留的巨噬细胞样黑色素细胞（PVM/MS）的变化。在6个月时可检测到毛细血管密度降低，在9至21个月大的小鼠中观察到显著的毛细血管变性。毛细血管密度降低与PC和PVM/MS数量减少高度相关。6个月时观察到PC的“脱落”和PVM/MS的“激活”，21个月时观察到剧烈变化。通过新建立的体外3D细胞共培养模型，我们证明了PC和PVM/MS对于维持耳蜗血管结构和稳定性至关重要。
Tight control over the cochlear blood flow (CoBF) and blood-labyrinth barrier (BLB) in the stria vascularis is critical for maintaining the ionic, fluid, and energy balance necessary for hearing function. Inefficient CoBF and disruption of BLB integrity have long been considered major etiologic factors in a variety of hearing disorders. In this study we investigated structural changes in the BLB of the stria vascularis in age-graded C57BL/6 mice (1 month to 21 months) with a focus on changes in two blood barrier accessory cells, pericytes (PCs) and perivascular-resident macrophage-like melanocytes (PVM/Ms). Decreased capillary density was detectable at 6 months, with significant capillary degeneration seen in 9 to 21 month old mice. Reduced capillary density was highly correlated with lower numbers of PCs and PVM/Ms. “Drop-out” of PCs and “activation” of PVM/Ms were seen at 6 months, with drastic changes observed by 21 months. With newly established in vitro 3D cell-based co-culture models we demonstrated PCs and PVM/Ms are essential for maintaining cochlear vascular architecture and stability.