Identification of a<i>Prunus</i>MAX1 Homolog as a Unique Strigol Synthase from Carlactone Bypassing 5-Deoxystrigol
Identification of a<i>Prunus</i>MAX1 Homolog as a Unique Strigol Synthase from Carlactone Bypassing 5-Deoxystrigol
复制标题
鉴定<i>Prunus</i>MAX1同源物作为绕过 5-Deoxystrigol 的 Carlactone 中的独特 Strigol 合酶
DOI：
10.1101/2022.10.24.513630
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发表时间：
2022
期刊：
影响因子：
9.4
通讯作者：
Li Yanran
中科院分区：
文献类型：
--
作者：
Wu Sheng;Zhou Anqi;Hiugano Kozue;Yoda Akiyoshi;Xie Xiaonan;Yamane Kenji;Miura Kenji;Nomura Takahito;Li Yanran
Strigol是第一个被发现的Strigolacone(SL)，但生物合成途径仍然难以捉摸。在这里，通过利用微生物SL产生平台进行快速基因筛选，我们在功能上鉴定了李中的Strigol合成酶(PpMAX1c，一种细胞色素P450 711a酶)，它通过催化多步氧化和C环环化直接从SL前体卡内酯合成Strigol，而不是合成5-脱氧Strigol。通过重组烟草中Strigol的生物合成，验证了PpMAX1c的功能。进一步的基因组分析和功能验证证实，桃还编码一种Orobancol合成酶(PpCYP722C，一种细胞色素P450 722C酶)，暗示桃中同时存在Strigol型和Orobancol型SLS，并通过桃苗的代谢分析得到证实。这项工作突出了很大程度上未被探索的CYP711A同系物家族的催化多样性，并为表征不同类型的SLS在经济上重要的李子中的作用奠定了基础。
Strigol was the first strigolactone (SL) to be discovered, but the biosynthetic pathway remains elusive. Here, through rapid gene screening using a microbial SL-producing platform, we functionally identified a strigol synthase (PpMAX1c, a cytochrome P450 711A enzyme) in Prunus that synthesizes strigol directly from the SL precursor carlactone through catalyzing multi-step oxidations and C-ring cyclization, bypassing the synthesis of 5-deoxystrigol. The function of PpMAX1c was validated through reconstructing the biosynthesis of strigol in Nicotiana benthamiana. Additional genomic analysis and functional verification confirm that peach also encodes an orobanchol synthase (PpCYP722C, a cytochrome P450 722C enzyme), which hints at the presence of both strigol-type and orobanchol-type SLs in peach and was confirmed through metabolic analysis of peach seedlings. This work highlights the catalytic diversity of the largely unexplored family of CYP711A homologs and sets the foundation to characterize the roles of different types of SLs in the economically important Prunus.